Protein Purification: Chromatography

Question 0

Equipment for liquid chromatography

Answer 0

• refrigeration
• buffer reservoir
• pump and gradient maker
• way to apply protein sample to column
• detection and monitoring system
• fraction collector

Question 1

Types of chromatography

Answer 1

Affinity
Ion exchange
Hydrophobic interaction
Gel filtration

Question 2

Definition of amphoteric

Answer 2

Net surface charge changes as pH changes

Question 3

Steps in gradient elution

Answer 3

Equilibration
Sample injection volume
Gradient elution
Wash
Re-equilibration

Question 4

What is kav?

Answer 4

A partition coefficient related to the size of a molecule

Globular proteins have a partially linear relationship between kav and logMr