Basic Principles Of Microscopy

Question 0

In phase …. Amplitude

Out if phase …. Amplitude

Answer 0

Increases

Decreases

Question 1

Abbes law

Answer 1

The minimum resolving distance is related to the wavelength of light divided by the numerical aperture, which is proportional to the angle of the light cone formed by a point on the object, to the objective

Question 2

Components of a microscope

Answer 2

Recording system
Eyepiece
Objective lenses
Specimen stage
Condenser lense
Light source
Epi-illumination source

Question 3

Types of electron microscopes

Answer 3

TEM = transmission electron microscope (thin)
SEM = scanning electron microscope (bulk)

Question 4

Role of the condenser lens

Answer 4

Focuses light onto the specimen

Fills the objective lens

Question 5

Describe kohler illumination

Answer 5

Creates an evenly illuminated field view while illuminating the specimen with a very wide cone of light

Question 6

How does differential interference contrast work?

Answer 6

Uses a beam splitter