Protein Fluorescence Flashcards
0
Q
Why do we use fluorescence
A
Provides info on molecular environment and on dynamic processes
1
Q
What is fluorescence
A
The light emitted by an atom or molecule after a finite duration subsequent to the absorption of electromagnetic energy
2
Q
Things that can be measured with fluorescence
A
- temp PH Viscosity Ions Polarity
3
Q
Instrumentation in fluorescence
A
Fluorimeters
Microscopes
High throughout plate readers
Intravital imaging
4
Q
What is the dominant intrinsic fluorophore?
A
Tryptophan
5
Q
3 ways to make a GFP fusion construct
A
Protein x - GFP
GFP - protein y
Protein -GFP - z
6
Q
Purpose of FRET
A
Fluorescence resonance energy transfer
Allows the detection of molecule-molecule interactions in the nanometer range with light microscopy
7
Q
MDCK clues
A
Madin-Darby canine kidney polarized epithelial cells
