Protein Methods Flashcards
0
Q
Requirements for protein purification
A
An assay for the protein of interest
A reasonable source of that protein
1
Q
Value of protein purification
A
Can establish particular biological activity
Produce valuable biochemical reagents
Understand enzyme mechanisms and inhibitors
Create novel protein
Understand 3d structure
2
Q
Basic scheme of protein purification
A
- prep, extraction, clarification
- capture
- intermediate purification
- polishin
