Cell Culture Flashcards
0
Q
Classification by manner of growth
A
- adherent growths
- suspension cultures
- hanging drops
1
Q
Types of culture and cell lines
A
Primary cells
Continuous lines
Transformed cells
Hybridomas (fusion of lymohocytes and immortal cells)
2
Q
How to sub-culture
A
- remove media and treat cells with trypsin and EDTA
- inactivate trypsin with medium-containing serum
- excess protein titrates away protein
- cells vigorously re suspended in fresh medium and plated
