CyTOF

Question 0

How is the plasma created?

Answer 0

Using a radio-frequency-generated electromagnetic field in the plasma torch

Question 1

Basic steps in cytof

Answer 1

Sample introduction
Ionization
Mass analysis
Data acquisition

Question 2

3 chambers of the torch

Answer 2

Outer - argon plasma gas
Central - argon auxiliary gas
Inner - in the sample injector (transmits argon stream and sample aerosol from spray chamber to center of plasma)

Question 3

Things found inside the ion beam

Answer 3

Heterogenous mixture if argon ions, endogenous cellular ions, isotopic probe ions, neutral particles and photons

Question 4

Role of interface region

Answer 4

• reduces temp and pressure of incoming clouds
• focuses the ionic beam to the downstream ion optics
• accelerated and highly focused ion beam after the reducer

Question 5

Role of quadrupole

Answer 5

Removes unwanted low MW argon and endogenous cellular ions

Question 6

What does cytof signal depend on.

Answer 6

Target abundance
Probe performance
Instrument detection efficiency for the metal isotope tag

Question 7

Why use cytof?

Answer 7

• can stain up to 40 markers
• reduces the quantity of samples needed
• allows use of multiple tetramers
• can barcode cells