Basics Of Multiphoton, Superresolution And Structures Illumination Microscopy Flashcards
0
Q
SR-SIM
A
Structured illumination super resolution microscopy
1
Q
Examples of super-resolution methods
A
Structured illumination
- SR-SIM
Spatially patterned excitation
- STED
- RESOLFT
Localization methods
- STORM
- PALM
2
Q
STED
A
Stimulated emission depletion
- drive down to ground state with second dump pulse before molecule can fluoresce
3
Q
STORM
A
Stochastic optical reconstruction microscopy
4
Q
PALM
A
Photo activated localization microscopy
- image only some dye molecules at one time
5
Q
De convolution microscopy
A
Operates on principle of PSF
As one moves away from focal plane in which the object lies, the light from it will spread in a predictable manner
