Proper Sample Storage & Submission (1) Flashcards
Dr. Helmer
What is a CBC?
whole blood
represents unseen, non clotted sample, +/- anticoagulant
What is a chemistry comprised of?
plasma and serum
Differentiate plasma and serum
plasma: fluid fraction of whole blood obtained from an anticoagulated sample
serum: fluid fraction of whole blood obtained from a clotted sample
Serum = ______ - _______
plasma minus fibrinogen
List the tube order you would follow if you drew blood on an animal and needed a CBC and chemistry panel
citrate (blue) top
red top
green top
purple top
How can plasma/serum appearance change in a blood sample?
hemolysis
icterus
lipemia
Describe how the CBC changes with intravascular hemolysis
hemolysis - leads to free Hb because red blood cells are lysed
therefore, the RBC, HCT, and PCV will be decreased
MCHC falsely elevated
ghost erythrocytes counted as platelets
refractometric proteins become difficult to read
MCV unaffected
Describe how the chemistry changes with intravascular hemolysis
spectrophotometric interference and inhibition of chemical reactions
increased in intra-RBC analytes
CK increase (mild)
severe hemolysis - cause beta-globulin spikes
Describe how the CBC changes with lipemia
falsely increases HGB and subsequently MCHC
large lipid aggregates may be falsely counted as platelets, increasing PLT, or potentially leukocytes, increasing WBC
falsely increase refractometric protein
Describe how the chemistry changes with lipemia
spectrophotometric interference
proportional decrease in Na and Cl, slight decrease in K
Describe how the CBC changes with icterus
little to no effect
Describe how the chemistry changes with lipemia
marked to severe hyperbilirubinemia falsely decreases biuret total protein and creatinine, respectively
