Pre-mat Proteins

Question 1

structure of amino acid

Answer 1

amino group

carboxyl group

lives in ionized form

Question 2

Polar AAs- neg charged

Answer 2

• Aspartic Acid

- Glutamic Acid

Question 3

Polar AAs- pos charged

Answer 3

+arginine
+lysine
+histidine

Question 4

Polar AAs- neutral

Answer 4

asparagine
glutamine
serine
threonine
tyrosine

Question 5

Non polar AA

Answer 5

Alanine
GLycine
Valine
Leucine
Isoleucine
Proline
Phenylalanine
Methionine
Tryptophan
CYsteine

Question 6

Aromatic AAs

Answer 6

phenylalanine
tyrosine
tryptophan

Question 7

peptide bond

Answer 7

between carboxy adn amino terminus of AAs

planar

Question 8

isoelectric point

Answer 8

pI, the pH at which a particular molecule carries no net electical charge or is electrically neutral

the charge on each AA side chain is determined by Henderson-Hasselbach equation

pH = pKa + log [A-]/[HA]

Question 9

Levels of protein structure

Answer 9

Primary
Secondary: alpha helix, beta pleat sheet (can be antiparallel or parallel)
-secondary structure motifs: helix=turn-helix, BaB, leucine zipper, zinc finger
Tertiary: domains and folds
Quaternary

Question 10

enzyme mediated PTMs of proteins

Non-enzyme mediated

Answer 10

addition of hydrophobic lipid group for membrane localization

addition of cofactor for enzyme activity

addition of small chemical groups: mostly reversible phospho, acetylation

NON enzyme mediated: glycation (addition of reducing sugar ex hemoglobin A1C)

Question 11

consensus sequences

Answer 11

protein modifers recongize conserved sequences call “” and are able to phospho (or whatever) many diff proteins with this conserve sequence