polymerase chain reaction Flashcards
1
Q
why do you use in vitro gene cloning
A
make copies of a gene artifically
2
Q
explain the advantages of polymerase chain reaction
A
+ quick
+ automated
3
Q
explain the process of polymerase chain reaction
A
- heat the DNA to 95 degrees
- DNA strands are copied and then separated as high temperatures break hydrogen bonds
- cool to 55 degrees
- primer anneals to complementary bases on DNA. This provides a start point for DNA polymerase to bind
- heat it to 72 degrees
- optimum temperature for DNA polymerase to join nucleotides
- DNA polymerase extends the primers this forms template strands.
4
Q
what 5 things does PCR need
A
- DNA
- DNA polymerase
- free nucleotides
- thermocycler
- primer
