PH1123 Vitamins and Coenzymes Flashcards

1
Q

how does taking unnecessary vitamin supplements effect health ?

A

One in three women and one in four men in the UK take dietary supplements for health reasons. But a review of 14 trials of vitamin pills taken by 170,000 people found they increased the death rate by 6 per cent. While they offered no explanation as to what caused the deaths, they discovered that the supplements offered no protection against cancers of the gut.

The researchers, writing in The Lancet, estimate that for every one million people taking the supplements, 9,000 would die prematurely as a result. The figure takes account of the background level of premature death in the population.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

what’s an apoenzyme ?

A

the large protein molecule that forms the bulk of the enzyme molecule. In isolation it may be able to bind substrate but not able to catalyse its reaction

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Whats a co factor ?

A

non-protein molecule or metal ion that binds to the apoenzyme to form the holoenzyme. The cofactor may be bound at the active site or at a distant site on the apoenzyme.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Whats a holoenzyme ?

A

Holoenzyme – the catalytically active form of the enzyme.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Whats the substrate ?

A

Substrate – the substance upon which an enzyme acts

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Define a coenzyme

A

Definition: A non-protein organic compound, produced in living cells, which is involved in the activation of enzymes.
Co-substrates are altered during the course of the reaction and then dissociate from the enzyme. They are then regenerated by another enzymatic reaction.
Prosthetic groups remain bound to the enzyme but still need to be regenerated.

An enzyme is a protein that acts as a catalyst to increase the biochemical reaction rate without altering itself in the process, while a coenzyme is an organic non-protein molecule that is required by an enzyme to perform its catalytic activity.
Unlike enzymes, the coenzyme is often structurally altered during an enzyme-catalysed reaction.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Whats the definition of a vitamin ?

A

Definition: General term for any of several organic substances essential for normal metabolic processes and which, when absent in the diet, produce deficiency states as THEY ARE NOT PRODUCED NATURALLY BY THE BODY.
Many coenzymes are synthesised from dietary precursors - these dietary precursors are often vitamins.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

describe the differences between water soluble and lipid soluble vitamins

A

WATER SOLUBLE

readily excreted via kidney in urine

 required daily (in small
amounts)

e.g. Vitamins B & C

LIPID SOLUBLE

stored in the body
(usually in liver)

excessive intake can
cause toxicity

e.g. Vitamins A, D, E & K

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

state general information for B group vitamins

A

Originally thought to be a single vitamin but now understood to be multiple vitamins – all of which form coenzymes

Some can be synthesised within the body by intestinal flora (note: this is different to being produced by the body)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

State the sources, function and coenzyme produced by vitamin B1

A

Sources:
Found in cells therefore present in all whole natural foods e.g. whole cereal grains, beans, fruits and yeast

Coenzyme produced: Thiamine pyrophosphate (TPP)

Function:
Coenzyme to several enzymes in carbohydrate metabolism pathways (helping to release energy from food)
Also involved in conduction of action potentials in neurons and neuro-muscular transmission

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

What is the deficiency state of vitamin B1 and in whom is it most prevalent?

A

Deficiency state
Prevalent in eastern Asia (white rice contains little thiamine) and in chronic alcoholics (severely malnourished). Athletes and pregnant/lactating women may require supplements (increased carbohydrate consumption)

Beriberi can affect different organ systems
Wet beriberi affects the cardiovascular system and presents with cardiac failure, dyspnoea and oedema

Dry beriberi affects the peripheral nervous system and presents with peripheral neuritis, paralysis and wastage. A severe form of dry beriberi is known as Wernicke–Korsakoff syndrome and is characterised by paralysis of eye movement, unusual movements and impaired mental function.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

State the sources, function and coenzymes produced by vitamin B2

A

Name: Vitamin B2 [A.K.A. riboflavin]

Sources:
Dairy products, eggs, green vegetables and almonds

Flavin mononucleotide (FMN)
Flavin adenine dinucleotide (FAD)

Function:
Heterocyclic system acts as a H acceptor (or donor)
Coenzymes act as prosthetic groups on a family of mainly oxidoreductase enzymes known as flavoproteins
Involved in the metabolism of fats, carbohydrates and proteins
Also acts as a coenzyme in the processing of other vitamins (e.g. conversion of vitamin B6 into its coenzyme)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

What is the deficiency state of vitamin B2 ?

A

Deficiency state
Rare in developed countries due to fortified foods but common in developing countries (due to malnutrition)
SYMPTOMS INCLUDE INFLAMMATION OF THE MOUTH AND LIPS (STOMATITIS) WHICH IS SIMILAR TO PELLAGRA BUT WITHOUT THE WIDESPREAD SKIN LESIONS (PELLAGRA SINE PELLAGRA)
It can also reduce iron absorption leading to anaemia with the size and haemoglobin content of red blood cells remaining normal (normochromic normocytic anaemia)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

State the sources, function and coenzymes produced by vitamin B3

A

Name: Vitamin B3 [A.K.A. nicotinamide (niacinamide); niacin (nicotinic acid)]

Sources:
Meat, fish and nuts
Nicotinamide also used in acne treatments

Coenzymes produced:
Nicotinamide adenine dinucleotide (NAD)
Nicotinamide adenine dinucleotide phosphate (NADP

Function:
Important in oxidoreductase enzyme reactions: NAD(P)+ is an oxidising agent whilst NAD(P)H is a reducing agent
NADPH is important in anabolic processes (e.g. lipid and nucleic acid synthesis) whilst NAD+ is vital to catabolic processes (e.g. metabolism of energy sources like fatty acids and glucose)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

What is the deficiency state of vitamin B3

A

Deficiency state
Body not entirely dependant on dietary intake as nicotinic acid can be produced from dietary tryptophan in vivo
Deficiency common in areas in which maize is the principal foodstuff (as it is low in both nicotinic acid and tryptophan)
Pellagra – characterised by ‘dermatitis, diarrhoea and dementia’.
Chronic alcoholics also at risk of deficiency

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

What is the toxicity state of vitamin B3 ?

A

Toxicity state
Skin flushes, liver damage (mainly seen with niacin supplementation; nicotinamide may be given as alternative supplement)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

State the sources, function and coenzymes produced by vitamin B5

A

Name: Vitamin B5 [A.K.A. pantothenic acid]

Sources:
Dairy products, eggs, avocado, mushrooms
Present in the outer layers of whole grains- milling greatly reduces the pantothenic acid content

Coenzyme produced: coenzyme A

Function:
Coenzyme in the synthesis and oxidation of fatty acids
Involved in the oxidation of pyruvate as part of the Krebs cycle

18
Q

What is the deficiency state of vitamin B5 ?

A

Deficiency state

Extremely rare and as a result has not been studied thoroughly

19
Q

State the Name, sources, function and coenzymes produced by vitamin B6

A

Name: Vitamin B6 [A.K.A. pyridoxine, pyridoxal, pyridoxamine]

Sources:
Found in most foods of vegetable (pyridoxine) or animal (pyridoxal, pyridoxamine) origin
Interconvertible via their phosphates in vivo

Coenzyme produced: pyridoxal-5’-phosphate

Function:
Involved in tryptophan metabolism (vitamin B6 deficiency is a risk factor for vitamin B5 deficiency)
Also involved in the production of a number of neurotransmitters (e.g. dopamine, GABA)
Acts as a coenzyme for an extremely broad range of enzymes (approximately 4% of enzymes; >140 reactions)

20
Q

What is the deficiency state of vitamin B6 ?

A

Deficiency state
Rare as most diets contain adequate amounts and some is synthesised by intestinal flora
Results in disorders of CNS, skin and mucous membranes
Patients taking medicines such as isoniazid may benefit from increased intake due to increased excretion of pyridoxine

21
Q

What is the toxicity state of vitamin B6 ?

A

Toxicity state
Supplementation with high doses of vitamin B6 can lead to nerve damage (particularly in spinal ganglia) which manifests as pain/numbness in the extremities or in extreme cases difficulty with motor functions

22
Q

State the Name, sources, function and coenzymes produced by vitamin B7

A

Name: Vitamin B7 [A.K.A. biotin]

Sources:
Egg, avocado, yeast, fresh vegetables

Coenzyme produced: N/A [biotin acts as a coenzyme]

Function:
Required by several carboxylase enzymes that are involved in fatty acid synthesis, amino acid breakdown and glucose synthesis (gluconeogenesis)

23
Q

what is the deficiency state of vitamin B7 ?

A

Deficiency state(s)
Rare – severe deficiency never reported in healthy individuals eating a normal mixed diet
Presents with thinning hair, brittle nails, rashes and neurological symptoms
Pregnant/lactating women at risk of deficiency (clinical reason unknown)
Those eating lots of raw eggs are at risk due to avidin in the whites reducing the absorption of biotin

24
Q

State the Name, sources, function and coenzymes produced by vitamin B9

A

Name: Vitamin B9 [A.K.A. folic acid]

Sources:
Nuts, seeds, chickpeas, green vegetables
Folic acid is the vitamin that is most commonly added to ‘fortified’ foods such as flour

Coenzyme produced: tetrahydrofolate (THF)

Function:
THF is the coenzyme in enzymatic reactions that transfer hydroxymethyl (-CH2OH), formyl (-CHO) and methyl (-CH3) groups in a large number of reactions and significantly in the synthesis of amino acids and purine/pyrimidine bases in the formation of DNA

25
Q

What is the deficiency state of vitamin B9 ?

A

Deficiency state
Patients with malignant disease or who are pregnant/breastfeeding are at risk of deficiency due to increased folic acid demand. A number of medications (e.g. methotrexate) interfere with folic acid processing and so create a risk of deficiency
Folic acid deficiency during pregnancy is associated with low birth weight, premature birth and neural tube defects
Supplements are therefore given in pregnancy, leukaemia and drug-induced folate deficiency
Deficiency can cause megaloblastic anaemia (large red blood cells) due to faulty erythrocyte multiplication and maturation
Supplementation with folic acid may mask vitamin B12 deficiency which leads to damage to the nervous system

26
Q

State the Name, sources, function and coenzymes produced by vitamin B12

A

Name: Vitamin B12 [A.K.A. cobalamin]

Sources:
Animal food sources (meat, fish, milk, eggs)
Rare in common plant-based foods (some in fermented foods and seaweed)

Coenzyme produced: adenosylcobalamin and methylcobalamin (the body converts hydroxocobalamin and cyanocobalamin from the diet into these active forms)

Function:
Acts as a cofactor for methionine synthase which is involved in the production of THF from folic acid
Also acts as cofactor in molecular rearrangement reactions (e.g. metabolism of branched-chain amino acids) – particularly in the central nervous system

27
Q

What is the deficiency state of Vitamin B12 ?

A

Deficiency state
Deficiency cause pernicious anaemia – megaloblastic anaemia plus gastrointestinal (diarrhoea, loss of bladder control) and neurological symptoms (seizures, degeneration of spinal cord)
Folic acid supplementation can reverse the megaloblastic anaemia but will not reverse the other symptoms
Care must be taken with anaemic patients to balance intake of folic acid, cobalamin and iron

28
Q

State the Name, sources, function and coenzymes produced by vitamin C

A

Name: Vitamin C [A.K.A. ascorbic acid]

Sources:
Citrus fruits, guava, kiwi, broccoli, and Brussels sprouts
First ever clinical trial used citrus juice vs. scurvy (1747)

Coenzyme produced: N/A [ascorbic acid acts as a cofactor]

Function:
Most powerful reducing agent known to occur naturally in living tissue
Acts as a cofactor in the hydroxylation of proline to hydroxyproline (creating more OH groups for H-bonding) which gives the triple helix of collagen its strength

Ascorbic acid is important due to its ability to maintain metal ions (also cofactors) in the correct ionic state within an enzyme’s active site (e.g. Fe2+ in the active site of prolyl hydroxylase)
It also acts as cofactor in steroid synthesis, noradrenaline production and the conversion of folic acid to THF
It also has an important independent role within the body as an antioxidant- donating electrons to limit the damage caused by free radicals and oxidative species
It is also involved in immune system regulation (especially during infection)

29
Q

What is the deficiency state of vitamin C?

A

Deficiency state
Scurvy is characterised by swollen gums, bruising, haemorrhage, bone fracture, loose teeth, poor wound healing and anaemia
Supplements may benefit those not following a balanced diet (elderly, infirm), diabetics, pregnant and lactating women, heavy drinkers and smokers

30
Q

What is the toxicity state of vitamin C ?

A

Toxicity state

Stomach complaints

31
Q

State the Name, sources, function and coenzymes produced by vitamin A

A

Name: Vitamin A [A.K.A. retinol]

Sources:
Cod liver oil, butter, cheese, milk
Retinol can be synthesised from carotenoids (found in carrots and tomatoes)

Coenzyme produced: N/A [retinol does not form a coenzyme]

Function:
Retinol is converted into retinal and retinoic acid in the body
Retinal is further converted to 11-cis-retinal (using NADP+ as a coenzyme) which is a photosensitive prosthetic group essential for normal function of the retina
The retinal molecule undergoes isomerisation in the presence of a photon of the correct wavelength of light
It is regenerated via the RPE65 enzyme so that it can be used to detect light again
Retinoic acid is required for normal embryonic development, growth, development, immune system function and the maintenance of epithelial tissue (including skin- retinoic acid used as psoriasis/acne treatment)

32
Q

What is the deficiency state of vitamin A ?

A

Deficiency state
Still widespread in developing world. Symptoms include night blindness, dry/ hyperkeratotic skin, skin infection, corneal damage and blindness.
Supplements may be required in breast feeding women to compensate for infant’s supply and in patients unable to absorb or store lipids

33
Q

What is the toxicity state of Vitamin A ?

A

Toxicity state
Toxicity to unborn children (C/I using retinoic acid in pregnancy)
Hypervitaminosis A – excessive intake (usually supplements or diet (esp. liver) can lead to liver damage, bone pain, vision changes and death

34
Q

State the Name, sources, function and coenzymes produced by vitamin D

A

Name: Vitamin D [A.K.A. calciferol]

Sources:
Ergocalciferol is found in fungi sources (e.g. mushrooms) and cholecalciferol is found in animal sources (e.g. fatty fish, cod liver oil, egg yolk)
The body is not entirely dependent on dietary intake as vitamin D3 is formed under UV light from provitamin precursors (7-dehydrocholesterol) in the skin

Coenzyme produced: N/A [calciferol does not form a coenzyme]

Function:
Calciferol is converted into calcifediol in the liver before being further altered in the kidney to produce calcitriol
Calcitriol stimulates synthesis of specific proteins that act as Ca2+ carriers in bone and intestine increasing absorption of dietary Ca2+ and release of Ca2+ from bone (PO4- passively accompanies the Ca2+ movements)
Calcitriol maintains Ca2+ and PO4- at sites of new bone formation therefore is essential for proper formation of the skeleton

35
Q

What is the deficiency state of vitamin D ?

A

Deficiency state
Calciferol deficiency leads to rickets in children- characterised by distortion of the long bones of the legs and bones of the pelvis and spine
Calciferol deficiency in adults leads to osteomalacia (bone softening)
Rate of synthesis of vitamin D in the skin depends on exposure to UV light therefore night workers and miners traditionally prone to deficiency
Rate of synthesis of vitamin D in the skin also depends on skin pigmentation. Melanin protects skin cells by preventing UV light penetration into the skin- this reduces the efficiency of the conversion of 7-dehydrocholesterol

36
Q

What is the toxicity state of vitamin D ?

A

Toxicity state

Hypercalcaemia  calcium deposits in organs (e.g. kidneys, liver, heart), anorexia, insomnia, abnormal bone formation

37
Q

State the Name, sources, function and coenzymes produced by vitamin E

A

Name: Vitamin E [A.K.A. the tocopherols and tocotrienols]

Sources: Vegetable oils (e.g. sunflower oil, rapeseed oil and palm oil)

Coenzyme produced: N/A [tocopherols/tocotrienols do not form coenzymes]

Function:
Act as a fat-soluble antioxidant – they are particularly important in protecting cell membranes from oxidative damage by free radical species
Also involved in smooth muscle growth and the maintenance of nerves

38
Q

What is the deficiency state of vitamin E ?

A
Deficiency state(s)
Usually due to malabsorption of fat rather than lack of dietary intake
Symptoms include nerve damage and haemolytic anaemia – due to oxidative damage to red blood cells
39
Q

What is the toxicity state of vitamin E ?

A

Toxicity state

Antagonises vitamin K- leading to risk of bleeding

40
Q

State the Name, sources, function and coenzymes produced by vitamin K

A

Name: Vitamin K [A.K.A. phytomenadione (K1), menaquinone (K2)]

Sources:
Leafy green vegetables (e.g. spinach, cabbage, kale) contain high levels of phytomenadione. Menaquinone is found in eggs, meat and dairy products. Intestinal flora can convert phylloquinone into menaquinone

Coenzyme produced: N/A [phytomenadione acts as a cofactor]

Function:
Acts as a cofactor in reactions that add a second carboxylic acid group onto the glutamate residues of a number of proteins to form a gamma-carboxyglutamate (Gla) residue
This is important in the formation of coagulation factors II (prothrombin), VII, IX, and X in the liver
Also involved in the production of anticoagulant proteins C and S and protein Z
Warfarin inhibits phytomenadione’s effects on the coagulation to mediate its clinical effect

41
Q

What is the deficiency state of vitamin k ?

A

Deficiency state
Rarely due to lack of dietary intake
Liver damage (e.g. alcoholics) and medication (e.g. anticoagulants) can lead to deficiency
Symptoms include anaemia, bruising and bleeding at the mucosal membranes (e.g. gums, nose)

42
Q

What is the toxicity state of vitamin K ?

A

Toxicity state
Unlike most fat-soluble vitamins, not stored in great quantities in the liver
Therefore, toxicity is not seen with higher doses of vitamin K1 or K2 in otherwise healthy people