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Structural Biochemistry > Peptide Sequencing > Flashcards

Peptide Sequencing Flashcards

1
Q

methods to determine primary structure?

A

chemical- edman deg
physical- MS
biological- DN sequencing, next gen seq

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2
Q

how does chemical peptide cleavage occur?

A

cyanogen bromide

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3
Q

how does enzymatic peptide cleavage occur?

A

trypsin or chymotrypsin

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4
Q

edman method of protein sequencing

A

disulfide bonds reduced - GluHCL

sanger reagent

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5
Q

advantages of MS over Edman

A

more sensitive, faster
doesnt require pure proteins
identifies blocked/mod proteins

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Structural Biochemistry (11 decks)

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