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Structural Biochemistry > Fluorescence > Flashcards

Fluorescence Flashcards

1
Q

who discovered fluorescence?

A

George Stokes

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2
Q

how does fluorescence occur?

A

photon absorption and molecules excitation

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3
Q

what is required for fluorescence?

A

large energy gap between S1 and S0 states
rigid aromatic molecular structure
repeatability

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4
Q

do heavy atoms increase or decrease fluorescence?

A

decrease

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5
Q

what are the main features of fluorescence?

A
  1. mirror rule- flu resembles abs
  2. stokes shift- emission shifted to longer wavelength
  3. shape - indep of lambda exc
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6
Q

what is the emission yield?

A

ratio of photons emitted and photons absorbed by fluorophore

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7
Q

what types of molecules are fluorescent?

A

proteins - tyr trp
nadh/fad
bilirubin, riboflavin
porphyrins, chlorophylls

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8
Q

what types of molecules are non fluorescent?

A

DNA/RNA

NAD/ NADP/ FADH

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9
Q

What flu stain is used on DNA?

A

DAPI, Hoechst (live)

Ethidium bromide, sybr safe (electrophoretic)

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10
Q

advantages of fluroescence?

A

high sensitivity/ selectivity
imaging
multi-color
intrinsic communication

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Structural Biochemistry (11 decks)

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