Nucleotide Isolation Flashcards
1
Q
NA isolation methods?
A
differential solubility
affinity methods
ultra centrifugation
2
Q
how is phenol used to isolate na?
A
it denatures proteins
3
Q
how is genomic DNA isolated?
A
- cell lysed: buffer & proteinase K
- phenol - chloroform extraction
- etoh pptn
4
Q
how does ethanol ppt Na?
A
less polar than water, weakens NA hydration
5
Q
which is more stable: DNA or RNA?
A
dna - rnase inhibitors
6
Q
NA isolation kits
A
adsorption on silica membranes
7
Q
how is mitochondrial DNA isolated?
A
magnetically- antibodies against receptor subunit
8
Q
DNA lambda max?
A
280nm
