Enzyme Kinetics Flashcards
1
Q
uses of enzyme kinetics?
A
- characterize new enzymes, activity, inhibitors
- screening compound and cell libraries
- measuring activity, sub conc, inhib conc
2
Q
enzyme bioassay set up
A
stock solutions of enzyme and substrate
buffer
additives (cofactor, ions)
reaction stop agent
3
Q
why do we screen enzyme libraries?
A
potential inhibitors/drugs, recomb enzymes
4
Q
enzyme inhibitor drugs?
A
statins, hypertension
5
Q
what is an enzyme inhibitor?
A
decreases rate of enzyme catalysed reaction
6
Q
how does an enzyme inhibitor work?
A
influences the binding of S, turnover number, or total conc of active enzymes
7
Q
what is a stopped enzyme assay?
A
reaction occurs for a set time period, amount of product is determined
8
Q
how is an enzyme assay stopped?
A
denaturation, ie acid/base, heat, inhibitors, EDTA, cold
9
Q
benefits of point of care testing
A
small sample, minimally invasive, rapid results, fast turnaround, reduced costs
