HPLC

Question 1

Who discovered chromatography?

Answer 1

M. Tswett (plant pigments)

Question 2

what does the performance of separation depend on?

Answer 2

selectivity
efficiency
capacity

Question 3

what effect does flow rate have on separation?

Answer 3

low flow rate - high diffusion

Question 4

what are the separation mechanisms?

Answer 4

dipole-dipole
hydrophobic
ionic
porous penetration
affinity interactions

Question 5

what interaction are in normal phase hplc?

Answer 5

dipole dipole, non polar to polar

Question 6

what interaction is in reversed phase chrom?

Answer 6

hydrophobic, polar to non polar

Question 7

FPLC mechanisms?

Answer 7

<50 bar pressure
separate proteins
medium plastic and glass columns

Question 8

LC Mechanisms?

Answer 8

larger beads
low pressure 1-5 bars
gravity flow

Question 9

HPLC mechanisms?

Answer 9

small beads
adsorption chromatography
high pressure (400 bar)
stainless steel column

Question 10

HPLC components

Answer 10

solvent, pump, injector, column, detector

Question 11

what are the elution modes?

Answer 11

isocratic - same eluent
linear/concave
step gradient

Question 12

what types of detection are used in HPLC?

Answer 12

photometric (UV-VIS)
refractometric
fluorescent
interfacing