Fluorescent Proteins Flashcards
1
Q
what does SAR stand for?
A
structure activity relationship
2
Q
how does bioluminescence occur in aequorea victoria?
A
blue 470 nm, Ca 2+ cofactor reaction passes energy to GFP, green 510 nm
3
Q
structure of GFP
A
238 AA peptide from jellyfish
central a helix, 11 antiparallel B sheets
central chromopore
4
Q
what wavelength do GFP abs and flu?
A
abs 475 nm (blue)
flu 510 nm (green)
5
Q
where does the flu in GFP come from?
A
Ser65-Tyr66-Gly67 post trans mod
6
Q
how does GFP flu?
A
post trans mod- tyr oxid, NH from Gly to Ser, deproton tyr H transfer to His, requires O2
7
Q
GFP variants
A
S65T: improved spectral properties
F64L: improved folding
8
Q
red flu proteins
A
corals, abs green, flu red
9
Q
what is FRET?
A
forster resonance energy transfer
Donor and Acceptor
dipole dipole coupling
