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PID > Pasteurella > Flashcards

Pasteurella Flashcards

1
Q

How do you define the serotypes of Pasteurella?

A

Capsule antigens

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2
Q

Where are Pasteurella usually commensals of?

A

URT

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3
Q

What gram stain are Pasteurellas?

A

Gram -ve coccobacilli

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4
Q

What is haemolysis of Pasteurellas?

A

Mainly haemolytic

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5
Q

What oxygen conditions will Pasteurella grow in?

A

Facultative anaerobes

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6
Q

What happens if you stain Pasteurella with Giemsa stain?

A

Ends stain best so get a bipolar appearance

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7
Q

How is Pasteurella capsule expression regulated in the animal??

A

By Fe2+

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8
Q

What is Pasteurella capsule like in vitro?

A

Heavily encapsulted

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9
Q

What is Pasteurella capsule made of?

A

Hyaluronic acid

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10
Q

Which endotoxin does Pasteurella cell wall contain?

A

LPS

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11
Q

Through which pathway does LPS act as an inflammatory mediator?

A

COX and NOS

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12
Q

Which substances regulate adhesion to the epithelium in Pasteurella?

A

OMPs and adhesins - ompA and oomph, porin, transferrin binding protein

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13
Q

How do you grow Pasteurella in culture?

A

Difficult - need sheep blood agar at 37

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14
Q

What is catalase and oxidase like in Pasteurella?

A

+ve

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15
Q

Where is the reservoir for Pasteurella?

A

The animal

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16
Q

What usually triggers Pasteurella outbreak?

A

Triggered by transport or management stress

17
Q

How do you get a new Pasteurella infection?

A

Inhalation or direct contact

18
Q

Which Pasteurella can you vaccinate sheep for?

A

P haemolytica and trehalosi, along with Clostridia

19
Q

Which Pasteurella can you vaccinate cattle for?

A

M haemolytica type 1

20
Q

Which Pasteurella can you vaccinate pigs for?

A

P multocida and Bordetella

21
Q

What is Actinobacillus gram staining like?

A

Gram -ve coccobacilli

22
Q

What is Actinobacillus oxygen culture like?

A

Aerobic but facultative anaerobic

23
Q

What do Actinobacillus colonies look like?

A

Mucoid 1-2mm, haemolytic

24
Q

Where is Actinobacillus found in the body?

25
Q

How do you tell Pasteurella, Actinobacillus and Haemophilus apart?

A

API strips (sugar fermentation), nutrients required for growth (X and V factor) and genetic/molecular typing

26
Q

What is gram staining of Haemophilus?

A

Small, motile gram -ve coccobacilli

27
Q

What is haemolysis of Haemophilus like?

28
Q

Where in the body if Haemophilus found?

29
Q

Which factors are required in Haemophilus?

A

X and V, although this varies between species

PID (21 decks)

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