Pasteurella Flashcards
How do you define the serotypes of Pasteurella?
Capsule antigens
Where are Pasteurella usually commensals of?
URT
What gram stain are Pasteurellas?
Gram -ve coccobacilli
What is haemolysis of Pasteurellas?
Mainly haemolytic
What oxygen conditions will Pasteurella grow in?
Facultative anaerobes
What happens if you stain Pasteurella with Giemsa stain?
Ends stain best so get a bipolar appearance
How is Pasteurella capsule expression regulated in the animal??
By Fe2+
What is Pasteurella capsule like in vitro?
Heavily encapsulted
What is Pasteurella capsule made of?
Hyaluronic acid
Which endotoxin does Pasteurella cell wall contain?
LPS
Through which pathway does LPS act as an inflammatory mediator?
COX and NOS
Which substances regulate adhesion to the epithelium in Pasteurella?
OMPs and adhesins - ompA and oomph, porin, transferrin binding protein
How do you grow Pasteurella in culture?
Difficult - need sheep blood agar at 37
What is catalase and oxidase like in Pasteurella?
+ve
Where is the reservoir for Pasteurella?
The animal