Parasitology Flashcards
Lab identification of Ancyclostoma duodenale and Necator americanus is made by observing ____ in fresh stool. On occasion, _____ worms may be seen attached to intestinal lining.
eggs; adult worms
A. duodenale adult worms can be differntiated from N. americanus by _____
A. duodenale has a pair of teeth; N.americanus has a pair of cutting plates
What is the diagnosis of choice for E. histolytica
EIA
This amoeba creates “flask-like lesions” in biopsies
E. histolytica
This amoeba has a “cigar-shaped” cromatoid body
E. histolytica
This amoeba contains up to 4 nuclei in its cyst form
E. histolytica
What is the cyst size and troph size of E. histolytica
Cyst: 12-15 um
Trop: 15-20 um
The nucleolous inside the nucleus = what (amoeba)
Karyosome
This amoeba looks like E.histolytica but is half the size
E. hartmanni
E. dispar is identical to E.histolytica and must be distinguished using DNA sequencing
True
What is the infective form of Ancanthamoeba
Cyst AND troph
This amoeba has a double walled cyst and has a “bulls-eye” nucleus in the troph
Acanthamoeba
Cryptosporidium, cyclospora, and cystoisospora are all groups of GI ______
Sporozoa/Coccidia
What GI sporozoa caused the waterborne outbreak in Milwaukee, 1994?
Cryptosporidium
What is the infectious form of sporozoa – cryptosporidium, cyclospora?
Oocyst
What is the test of choice for Cryptosporidium?
EIA
What stain is used to diagnose Cryptosporidium?
What secondary test used to diagnose Cryptosporidium?
Acid-fast; DFA
What is the test of choice for Giardia?
What is a secondary test used?
EIA; String test
What is the size of the Cryptosporidium oocysts> What is distinguishing features?
4-6um & crescent-shaped sporozoites
How big is Cyclospora oocysts?
8-10um
Can cryptosporidium or cyclospora be transmitted human to human?
Cryptosporidium NOT cyclospora
What stains can be used to ID Cyclospora?
MAF or Modified Safrinin
MS is usually better; less “ghosts” compare to MAF
What sporozoa/coccidia autofluoresce?
Cyclospora; Cystoisospora belli