Anaerobes Flashcards
What colonies will produce “brick-red” fluorescence under long wave UV light on KVLB agar?
Prevotella
What colonies will produce chatareuse (green) fluorescence under long wave UV light on KVLB agar?
Fusobacterium, excluding F. nucleatum (will not grow on KVLB agar)
What anaerobes cause foodborne illnesses?
C. botulinum and C. perfringes
What is the ONLY genus of anaerobes that will produce spores?
Clostridium
What are the two toxins C. perfringes produces?
Alpha toxin (lecithinase) and beta toxins
*Think: double zone of hemolysis = two types of toxins
What anaerobe produces small rods with terminal spores giving a drumstick apperance
C. tetani
What organism produces neurotoxin tetanopasmin
C. tetani
What organism causes pseudomembranous colitis?
Clostridium difficile
What are the two toxins C.difficile produces?
Toxin A & Toxin B
What are the major risk factors for C.difficile?
Antibiotic exposure and hospitalization/institutionalization
What is the “gold standard” test for C.diff?
Cytotoxicity assay
What organism has “horse manure” smell?
C.diff
Cycloserine cefoxitin fructose agar is used to isolate which organism?
C.diff
Veillonella is a gram negative _____
cocci
Gemella is a gram positive ____
cocci