Biochemical ID Flashcards
Phenylalanine deaminase will turn the tube what color when positive?
Yellow
What organisms are phenylalanine deaminase positive?
Proteus, Providencia, Morganella
The mixed acid fermentation process will result in lots of ______ (gas/acid) production. What test is this?
Acid; Methyl red
The butylene glycol fermentation pathway results in lots of _____ (gas/acid) production. What test is this?
CO2 gas; Voges-Proskauer
What does the Voges-Proskauer test test for?
The ability of bacteria to ferment glucose to the non-acid product butanediol
What is the VP reaction?
glucose → [acetoin] → butanediol VP
What is the indicator for VP test?
40% KOH and α-naphthol
What will the TSI rxn be for Shigella?
K/A (Alk/A) – does NOT ferment sucrose
What will the TSI rxn be for Salmonella?
K/A – Does NOT ferment sucrose
1) What will the TSI rxn be for Yersinia?
2) What will the KIA rxn be for Yersinia?
Why?
A/A – ferments sucrose in TSI
K/A – Does not ferment glucose in KIA
What is the purpose of sodium thiosulfate
Source of sulfur atoms for hydrogen sulfide production
What are the colors of E. coli colonies on Eosin methylene blue agar?
Green-black metallic sheen
What are the colors of weak lactose fermenters on Eosin methylene blue agar?
Purple
What are the colors of non-lactose fermenters on Eosin methylene blue agar?
Transparent
What decarboxylase rxn is used to differentiate b/w Shigella A, B, C, D?
Ornithine – Shigella sonnei (d) is the only one positive
What orgs are urease positive?
PPM-K – proteus, providencia ret, morganella, klebsiella
**Only PRET = urease positive
What orgs are citrate positive?
Entero-Citro-Se-Pr-K
Enterobacter, Citrobacter, Serratia, Proteus, Kleb
What are the lysine, arginine, and ornithine rxns for Salmonella
Positive for all – Tube will be purple + black
What are the lysine, arginine, and ornithine rxns for Enterobacter aerogenes?
+, -, +
What are the lysine, arginine, and ornithine rxns for Enterobacter cloacae?
-, +, +
What color indicates a positive rxn for lysine, arginine, and ornithine rxns
Purple
What orgs are H2S positive
Salmonella, Citrobacter, Proteus, Edwardsiella
What orgs are non-motile
Shigella and Klebsiella
What orgs are indole positive
Ecoli, KOXY, Edwardsiella, Morganella, Proteus vulgaris, Providencia, Pleisimonas
What Citrobacter produces H2S?
Citrobacter freundii
What Citrobacter produces indole?
C. koseri
What is the main way to distinguish between Salmonella and Citrobacter freundii?
LYSINE
lysine (+) in Salmonella
lysine (-) in CFREU
Serratia can be distinguished from Enterobacter because Serratia is DNAase ______ (pos/neg)
Positive
In the test for urease production, ammonia reacts
to form which product?
Ammonium carbonate
