HACEK Group + Neisseria

Question 1

What color indicates a positive aminolevulinic acid - porphyrin test?

Answer 1

PINK

Question 2

What organisms (3) are aminolevulinic acid positive?

Answer 2

H. parainfluenzae, H. parahaemolyticus, H. aphrophilus

Question 3

This test differentiates the hemin producing strains of Haemophilus

Answer 3

Aminolevulinic acid porphyrin (ALA)

Question 4

H. parainfluenzae is ____ positive and ____ negative (NAD/HEMIN)

Answer 4

NAD (V) positive, Hemin negative (X)

Question 5

What organism produces a slight yellow pigment with “grade-school paste” odor?

Answer 5

Haemophilus/Aggregatibacter aphrophilus/paraphrophilus

Question 6

What HACEK org is indole POSITIVE and makes a rosette pattern on gram stain

Answer 6

Cardiobacterium hominis

Question 7

What organism creates pitting of the agar and has a bleach smell?

Answer 7

Eikenella corrodens

Question 8

This organism causes “clenched fist” and “skin popping” infections

Answer 8

Eikenella corrodens

Question 9

What HACEK orgs are oxidase pos and neg?

Answer 9

Haemophilus aphrophilus and Actinobaccilus= OXIDASE NEGATIVE

Cardiobacterium, Eikenella, Kingella = OXIDASE POSITIVE

Question 10

What HACEK org causes agar pitting and JOINT damage

Answer 10

Kingella

Question 11

Which organism shows fusiform gram negative rods on gram stain and has (gliding motility) “sweaty-fingerlike projections” on BAP?

Answer 11

Capnocytophaga

Question 12

Capnocytophaga canimorsus and C. cynodegmi are associate with what type of infections?

Answer 12

Dog/cat bites

NOFL in dogs and cats

Question 13

The best specimen for Bordatella pertussis is?

Answer 13

Nasopharyngeal swab

Question 14

Bordatella pertussis is biochemically inert. How is it identified?

Answer 14

DFA - fluorescent antibodies

Question 15

Bordatella pertussis is oxidase ______ (pos/neg), _____ (motile/non-motile)

Answer 15

oxidase positive, nonmotile

Question 16

Bordatella ________ causes a much less severe version of pertussis

Answer 16

B. parapertussis

Question 17

B. parapertussis is oxidase _______

Answer 17

negative

Question 18

B. parapertussis grows on BG, RL, and _____

Answer 18

Chocolate

Question 19

Bordatella bronchiseptica causes what in animals?

Answer 19

Kennel cough in dogs/cat

Question 20

These guys dont grow on BAP because they release NADase in agar which inactivates NAD but will grow on horse or rabbit agar which contain no NADase

Answer 20

H. influenzae

Question 21

Non-motile, small pleomorphic coccobacilli

Answer 21

H. influenzae

Question 22

What organism causes Waterhouse-Friederchen syndrome?

Answer 22

Neisseria meningitis

Question 23

What gram negative diplococci has “hockey puck” colonies that move over surface?

Answer 23

Moraxella catt

Question 24

Which org is DNAse positive?

a. Moraxella catarhalis
b. NGON
c. NMEN
d. N. lactima

Answer 24

Moraxella catarhalis

Question 25

What org is superoxol positive? (HINT: This is the catalase test using 30% H2O2)

a. Moraxella catarhalis
b. NGON
c. NMEN
d. N. lactima

Answer 25

NGON! This test is used to differentiate b/w other Neisseria species