HACEK Group + Neisseria Flashcards
What color indicates a positive aminolevulinic acid - porphyrin test?
PINK
What organisms (3) are aminolevulinic acid positive?
H. parainfluenzae, H. parahaemolyticus, H. aphrophilus
This test differentiates the hemin producing strains of Haemophilus
Aminolevulinic acid porphyrin (ALA)
H. parainfluenzae is ____ positive and ____ negative (NAD/HEMIN)
NAD (V) positive, Hemin negative (X)
What organism produces a slight yellow pigment with “grade-school paste” odor?
Haemophilus/Aggregatibacter aphrophilus/paraphrophilus
What HACEK org is indole POSITIVE and makes a rosette pattern on gram stain
Cardiobacterium hominis
What organism creates pitting of the agar and has a bleach smell?
Eikenella corrodens
This organism causes “clenched fist” and “skin popping” infections
Eikenella corrodens
What HACEK orgs are oxidase pos and neg?
Haemophilus aphrophilus and Actinobaccilus= OXIDASE NEGATIVE
Cardiobacterium, Eikenella, Kingella = OXIDASE POSITIVE
What HACEK org causes agar pitting and JOINT damage
Kingella
Which organism shows fusiform gram negative rods on gram stain and has (gliding motility) “sweaty-fingerlike projections” on BAP?
Capnocytophaga
Capnocytophaga canimorsus and C. cynodegmi are associate with what type of infections?
Dog/cat bites
NOFL in dogs and cats
The best specimen for Bordatella pertussis is?
Nasopharyngeal swab
Bordatella pertussis is biochemically inert. How is it identified?
DFA - fluorescent antibodies
Bordatella pertussis is oxidase ______ (pos/neg), _____ (motile/non-motile)
oxidase positive, nonmotile
Bordatella ________ causes a much less severe version of pertussis
B. parapertussis
B. parapertussis is oxidase _______
negative
B. parapertussis grows on BG, RL, and _____
Chocolate
Bordatella bronchiseptica causes what in animals?
Kennel cough in dogs/cat
These guys dont grow on BAP because they release NADase in agar which inactivates NAD but will grow on horse or rabbit agar which contain no NADase
H. influenzae
Non-motile, small pleomorphic coccobacilli
H. influenzae
What organism causes Waterhouse-Friederchen syndrome?
Neisseria meningitis
What gram negative diplococci has “hockey puck” colonies that move over surface?
Moraxella catt
Which org is DNAse positive?
a. Moraxella catarhalis
b. NGON
c. NMEN
d. N. lactima
Moraxella catarhalis
What org is superoxol positive? (HINT: This is the catalase test using 30% H2O2)
a. Moraxella catarhalis
b. NGON
c. NMEN
d. N. lactima
NGON! This test is used to differentiate b/w other Neisseria species
