Mycobacterium

Question 1

The Non-MTB group consists of:

Answer 1

Photochromogens, Scotochromogens, and Non-photochromogens

Question 2

M. kansasii grows best at ____C.

Answer 2

35C

Question 3

M. marinum grows best at ____C (range)

Answer 3

28-32C

Question 4

What org will have a negative result for niacin, nitrate reduction, Tween hydrolysis (5days) and iron uptake?

Answer 4

M. bovis

Question 5

This group will show yellowish colonies in dark or light

Answer 5

Scrotochromogens

Question 6

This group will show yellowish colonies after exposure to light

Answer 6

Photochromogens

Question 7

What is the name of this test? Blood test for latent and active TB disease

Answer 7

QuantiFERON-TB Gold

Question 8

Name this test: blood test for exposure to bacteria

- can be positive from non-TB mycobacteria

Answer 8

Interferon-Gamma Release Assay (IGRA)

Question 9

What are the 5 first line drugs for TB

Answer 9

isoniazid, rifampin, ethambutol, pyrazinamide, streptomycin

Question 10

Processing stools cx for AFB will only occur if…?

Answer 10

Smear is POSITIVE

Question 11

Iron assimilation test is done to ID (fast or slow) growers?

Answer 11

Fast growers only – all slow growers = negative

Question 12

What BSL cabinet is required for Mycobacterium

Answer 12

BSL-3

Question 13

M. gordonae is nitrate ___ (+/-) and Tween hydrolysis (+/-)

Answer 13

Nitrate negative (-)
Tween hydrolysis (+)

Question 14

What scotochromogen is also know as “tap-water bacillus” and considered a contaminant

Answer 14

M. gordonae

Question 15

What scotochromogen is yellow/orange?

Answer 15

M. gordonae

Question 16

M. scrofulaceum nitrate ___ (+/-) and Tween hydrolysis (+/-)

Answer 16

Nitrate (-); Tween (-)

Question 17

This org is associated with contamination of hot water systems in hospitals and grows best at 42C

Answer 17

M. xenopi

Question 18

This org is the second most common cause of non-TB lung disease

Answer 18

M. kansasii

Question 19

What are the biochem rxns for M. kansasii

CAT, Niacin, Nitrate, and Tween

Answer 19

CAT +
Niacin -
Nitrate reduction +
Tween +

Question 20

What biochemical rxn differentiates between M. kansasii and MTB?

Answer 20

Niacin neg

Question 21

What are the biochem rxns for M. marinum

CAT, Niacin, Nitrate, and Tween

Answer 21

CAT -
Niacin -/+
Nitrate -
Tween +

Question 22

This org requires HEMIN and grows at 30C and 37C

Answer 22

M. haemophilum

Question 23

This org causes painless nodules under skin also known as Buruli’s ulcers

Answer 23

M. ulcerans

Question 24

What organism is the 1st most common cause of NTB lung disease?

Answer 24

M. avium/intracellulare complex

Question 25

Nocardia farcinica gives a _____ rxn for gelatin hydrolysis

Answer 25

Negative