parasitology Flashcards

1
Q

protozoan

A

unicellular eukaryotes

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2
Q

metazoan

A

multicellular

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3
Q

parasite life cycle

A

1.direct;
2.indirect;
3definitive or primary host:where parasite reaches maturity and undergoes sexual reproduction.
4;reservoir host: can harbour pathogen often with minimal effect.
5:secondary or intermediate host:where parasite usually undergoes asexual reproduction.

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4
Q

zoonosis

A

disease which can be transmitted to humans from animals(

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5
Q

anthroponosis

A

spread from human to human

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6
Q

parasite test

A
  1. fecal(stool examination)
  2. urine sampling–ova(malaria)
  3. CSF;
  4. blood test;
  5. invasive aspirates or biopsy;
  6. MRI
  7. PCR
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7
Q

blood test

A
  1. blood flim-good for high infection rate such as malaria or Africa trypanosomes
  2. blood culture-detect blood borne pathogens at lower infection rate such leishmania, candida or histoplasma
  3. For direct test-immunoassay to detect presence of pathogen antigens or ab or PCR to detect ag.
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8
Q

rapid diagnostic tests advantage

A
  1. easy, no special technical skills required
  2. cheap;
  3. sensitivity and specificity
  4. stable in poor enviornment;
  5. may need confirmation test
  6. can use a range of patient sample.
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9
Q

rapid diagnostic tests method

A

 1. The first step of the test procedure involves mixing the patient’s blood with a lysing agent in a test strip or well. This ruptures the red blood cells, releasing more parasite protein.
 2. Dye-labeled antibody, specific for target antigen, is present on the lower end of nitrocellulose strip or in a plastic well provided with the strip. Antibody, also specific for the target antigen, is bound to the strip in a thin (test) line, and either antibody specific for the labeled antibody, or antigen, is bound at the control line.

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