parasitology Flashcards
protozoan
unicellular eukaryotes
metazoan
multicellular
parasite life cycle
1.direct;
2.indirect;
3definitive or primary host:where parasite reaches maturity and undergoes sexual reproduction.
4;reservoir host: can harbour pathogen often with minimal effect.
5:secondary or intermediate host:where parasite usually undergoes asexual reproduction.
zoonosis
disease which can be transmitted to humans from animals(
anthroponosis
spread from human to human
parasite test
- fecal(stool examination)
- urine sampling–ova(malaria)
- CSF;
- blood test;
- invasive aspirates or biopsy;
- MRI
- PCR
blood test
- blood flim-good for high infection rate such as malaria or Africa trypanosomes
- blood culture-detect blood borne pathogens at lower infection rate such leishmania, candida or histoplasma
- For direct test-immunoassay to detect presence of pathogen antigens or ab or PCR to detect ag.
rapid diagnostic tests advantage
- easy, no special technical skills required
- cheap;
- sensitivity and specificity
- stable in poor enviornment;
- may need confirmation test
- can use a range of patient sample.
rapid diagnostic tests method
1. The first step of the test procedure involves mixing the patient’s blood with a lysing agent in a test strip or well. This ruptures the red blood cells, releasing more parasite protein.
2. Dye-labeled antibody, specific for target antigen, is present on the lower end of nitrocellulose strip or in a plastic well provided with the strip. Antibody, also specific for the target antigen, is bound to the strip in a thin (test) line, and either antibody specific for the labeled antibody, or antigen, is bound at the control line.