mycology Flashcards
superficial mycoses(tinea) caused by
- Trichophyton.spp
- Microsporum spp
- Epidermophyton spp
dermatophytes utilise _____
keratin
list sub-cutaneous mycoses
sporotrichosis(sporothix schenckii) chromoblastomycosis phaeohypohomycoses rhinosporidiosis lobomycosis
occasionaly occur in WA
S. Schenckii
endemic mycoses
known geographic distribution for each
valley river
histoplasma
usa
histoplasmosis,coccidioidomycosis,blastomycosis
direct microscopy procedures
- wet mount(w/m) :10%KOH+Calcofluor White (CW)
2. air dry smear&tissue specimens stained: PAS, GMS
clinical isolation media for fungal
SDA+/- chloramphenicol
SDA+chloramphenicol+cycloheximide
brain-heart infusion +5% sheep blood+ chloramphenicol
SDA+/- chloramphenicol use to
selecte fungi(selective media)
Media used to induce mould sporulation
PDA(potato dextrose agar)
potato carrot agar
Modified Borelli’s lachtrimel agar
czapek-dox agar
yeast identification(phenotypic )
germ tube test
assimilation test systems
cryptococcus identification
india ink preparation
mould identify
micro-broth dilution susceptibility test