PARASIT BASICS LAB Flashcards

1
Q

Most common fixative for preservation in Parasit

A

10% formalin
polyvinyl alcohol

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2
Q

All purpose fixative
has 1:3 stool to fixative ratio

A

formalin

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3
Q

Formalin concentration for protozoan cyst

A

5%

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4
Q

concentration of formalin for helminth eggs and larvae

A

10%

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5
Q

Very useful for field survey fixative
disadvantage contains mercuric chloride

A

Merthiolate Iodine Formaldehyde

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6
Q

Considered as the gold standard solution
permanent stain smear or execellent preservation of protozoan trophozoites and cysts

A

Schaudinn’s solution
Mercury based

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7
Q

Eco friendly fixative

A

Modified polyvinyl alcohol
copper or zince

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8
Q

Macroscopic examination includes

A

color
consistency
adult worms, proglottids
pus
mucus, blood

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9
Q

Microscopic examination includes

A

Parasites and ova

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10
Q

Preparation for microscopic

A

direct wet preparation
concentrated technique (fixed/fresh)
Permanently stained smear

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11
Q

Ocular micrometer

A

disk inserted to the eyepiece
size is important
measured as microms

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12
Q

best technique for the recovery of Schistosoma, opeculated eggs, trematode eggs, cestode eggs

A

Sedimentation technique
FEACT

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13
Q

Dissolves neutral fats/lipids and CHO

A

ether/ethyl acetate

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14
Q

best technique for the recovery of Protozoan cyst, nematode

A

Flotation
main reagent: 33% zinc sulfate
spx: stool

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15
Q

specific gravity range in floatation in zinc sulfate solution

A

1.18-1.20

fresh stool: 1.18
fixed stool 1.20

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16
Q

Best floatation technique for cryptosporodium, cyclospora

A

Sheathers sugar floatation
not the method of choice for helminths

17
Q

modified trichrome color change

A

spores are pink to red green background

18
Q

egg counting procedure that uses sieved and wire mesh

A

Kato katz

19
Q

recommended method of WHO useful in assessing intensity of infection of STH and unholy three

A

Cellophane covered smear

20
Q

thin film for malaria

A

confirmatory
malaria id

21
Q

thick film for malaria

A

screening
quantifying malarial parasite

Thick and thin smear is the gold standard

22
Q

parasites recovered in blood

A

Leishmania donovani
Trypanosoma spp
Plasmodium
Babesia
Microfilariae

1hr if malaria is suspected

23
Q

CSF PARASITE

A

Naegleria fowleri and Acanthamoeba
Trypanosoma spp - sleeping disease

23
Q

Rectal biopsy specimen parasite

A

Schistosoma spp

24
Q

Skin biopsy skin snips

A

Onchocerca volvulus
Mansonella

25
Q

Sputum

A

ASH
Ascaris
Strongyloides
Hookworm

26
Q

Eye specimens

A

Acanthamoeba keratitis
T. gondii
Loa loa

27
Q

Mouth scrapings

A

Entamoeba gingivalis
Trichomonas tenax

28
Q

Nasal discharge

A

Naegleria fowleri

29
Q

Gold standard for parasitic examniation diseases

A

Microscopic examination

30
Q

when fixative is not an option, stool can be mixed in ________

A

Potassium dichromate 1:1
Absolute ethanol 1:1

stored at 4C frozen with dry ice

31
Q
A