PARASIT BASICS LAB Flashcards
Most common fixative for preservation in Parasit
10% formalin
polyvinyl alcohol
All purpose fixative
has 1:3 stool to fixative ratio
formalin
Formalin concentration for protozoan cyst
5%
concentration of formalin for helminth eggs and larvae
10%
Very useful for field survey fixative
disadvantage contains mercuric chloride
Merthiolate Iodine Formaldehyde
Considered as the gold standard solution
permanent stain smear or execellent preservation of protozoan trophozoites and cysts
Schaudinn’s solution
Mercury based
Eco friendly fixative
Modified polyvinyl alcohol
copper or zince
Macroscopic examination includes
color
consistency
adult worms, proglottids
pus
mucus, blood
Microscopic examination includes
Parasites and ova
Preparation for microscopic
direct wet preparation
concentrated technique (fixed/fresh)
Permanently stained smear
Ocular micrometer
disk inserted to the eyepiece
size is important
measured as microms
best technique for the recovery of Schistosoma, opeculated eggs, trematode eggs, cestode eggs
Sedimentation technique
FEACT
Dissolves neutral fats/lipids and CHO
ether/ethyl acetate
best technique for the recovery of Protozoan cyst, nematode
Flotation
main reagent: 33% zinc sulfate
spx: stool
specific gravity range in floatation in zinc sulfate solution
1.18-1.20
fresh stool: 1.18
fixed stool 1.20