P2. Lowenstein Jensen (LJ) medium Flashcards
1
Q
original color of LJ?
A
light green
2
Q
LJ is selective for?
A
Mycobacterium spp.
3
Q
Selective inhibitors?
A
Malachite Green
(Inhibitor of Respiratory
Normal Flora)
4
Q
SAMPLE in LJ medium:
A
Sputum
5
Q
SPUTUM SAMPLE needs to be:
A
- Decongested/Digested
- Decontaminated
6
Q
DECONGESTED/ DIGESTED
To dissolve the thick mucus/mucin that might be trapping the bacteria in the sample. ____ is usually used.
A
N-acetyl-L-cysteine (NALC)
7
Q
Decontaminated.
To eliminate normal flora that contaminates the sample. ____ is usually used.
A
NaOH
8
Q
four (4) different groups of Mycobacterium:
A
- photochromogens
- scotochromogens
- non-photochromogens
- rapid growers
9
Q
contains pigments when incubated WITH light
A
photochromogens
10
Q
produce pigment when incubated WITHOUT light
A
scotochromogen
11
Q
do not produce pigment regardless of presence
or absence of light
A
non-photochromogen
