P2. 8 characteristics/ biochemical culture media.

Question 1

• semi-solid
• used to observe HYDROGEN SULFIDE GAS, INDOLE PRODUCTION, MOTILITY

Usually inoculated until the middle with the inoculating needle at 37°C for 18-24 hrs.

Answer 1

SIM Medium

or

Sulfide Indole Motility Medium

+ Motility has turbidity around the line of stab.

Question 2

detects bacterial pathogen that metabolize GLUCOSE using the MIXED ACID PATHWAY.

Answer 2

MR

or

Methyl Red

Question 3

detects bacterial pathogen that metabolize GLUCOSE using the BUTYLENE GLYCOL PATHWAY

Answer 3

VP

or

Voges-Proskauer

Question 4

detects bacterial pathogen that can utilize CITRATE as a SOLE SOURCE OF CARBON

Answer 4

SCA
or
Simmon-Citrate Agar

Question 5

Citrate was used when there is…

Answer 5

growth + bluing

Question 6

• Used for the determination of bacterial pathogen’s ability to ferment GLUCOSE, SUCROSE, or LACTOSE.
• can also detect SULFIDE & GAS PRODUCTION.

Answer 6

TSI
or
Triple Sugar Iron Agar

Question 7

• Used for the determination of bacterial pathogen’s ability to DECARBOXYLATE or DEAMINATE LYSINE.
• can also detect GLUCOSE FERMENTATION, SULFIDE PRODUCTION, & GAS PRODUCTION.

Answer 7

LIA

or
Lysine Iron Agar

Question 8

used to detect:
- LYSINE DECARBOXYLATION
- ORNITHINE DECARBOXYLATION
- ARGININE DIHYDROLYSIS

Answer 8

Moeller’s Broth

Question 9

used to detect bacterial pathogen that HYDROLYZE UREA SUBSTRATE

meron bang UREASE ENZYME?

Answer 9

Stuart’s Urea Broth

or

Christensen Urea Agar