Overview of Modern Blood Bank

Question 1

Who is the regulating agency for blood bank?

Answer 1

the FDA

Question 2

Fresh frozen plasma (FFP) must be prepared within ___ of collection of the whole blood unit.

Answer 2

8 hours

Question 3

Refrigerators maintained at ____

Answer 3

1-6 degrees celsius

Question 4

Freezers maintained at_____

Freezers maintained at _____

Answer 4

-18 and -65 degrees celsius

Question 5

Labeling of blood products occurs only after a careful review of all test results, including

Answer 5

No discrepancies in the ABO and Rh testing
Absence of detectable antibodies in plasma-containing components
Nonreactive viral marker tests
Nonreactive syphilis test

Question 6

What must a blood bank reconfirm if they receive blood from another center?

Answer 6

• reconfirm the ABO of each RBC-containing product

- Rh type of all Rh-negative units must be reconfirmed

Question 7

How many identifiers are needed for patient samples?

Answer 7

two

Question 8

What information is required for labeling a blood unit?

Answer 8

patient’s full name, DOB, and medical record number

Question 9

What tests are routinely done for a type and screen?

Answer 9

ABO and Rh

Question 10

When doing prenatal screening, what tests will be performed?

Answer 10

ABO, Rh, perhaps weak D and partial D testing, if antibody screen is positive the antibody must be identified.

Question 11

What is part of the postpartum evaluation?

Answer 11

all women are required to have their Rh status evaluated.

If mother is Rh negative and fetus is positive the mother is further evaluated for fetomaternal hemorrhage (FMH)

Question 12

What tests are performed for cord blood studies?

Answer 12

If the mother is Rh negative, cord blood will be tested for D and weak D.
ABO, Rh, and DAT will be performed on cord blood if the mother has significant anitbodies.

Question 13

What does A positive antibody screen in the absence of a positive autocontrol or DAT result indicate?

Answer 13

alloantibody immunization by means of blood transfusion or pregnancy

Question 14

Why might the DAT be positive?

Answer 14

• immune reaction to a drug
• disease
• delayed HTR

Question 15

How can you get rid of warm autoantibodies?

Answer 15

• Removal of RBC-bound autoantibody followed by serum autoadsorption
• Heterologous or differential serum adsorptions
• Elution techniques
• Autoantibody identification
• Cell separation techniques

Question 16

How can you get rid of cold autoantibodies?

Answer 16

• -Removal of RBC-bound autoantibody using 37°C saline
• -Prewarmed technique
• -Autoadsorption
• -Treatment of serum or cells with DTT or 2-ME
• -Adsorption using rabbit erythrocyte stroma