Antiglobulin testing

Question 1

“Complete” agglutinins that agglutinate red cells in saline are of which immunoglobulin class?

Answer 1

IgM

Question 2

What type of globulin does the antiglobulin test detect?

Answer 2

IgG alloantibodies
IgG autoantibodies
C3b complement components

Question 3

Why is incubation omitted in the direct AHG test?

Answer 3

In vivo antigen-antibody complex is already formed

Question 4

What do “check cells” contain?

Answer 4

Rh (D)+ red cells coated with anti-D

Question 5

Why does anti-C3d activity require standardization in AHG reagents?

Answer 5

high activity will fix anti-I and other clinically insignificant antibodies

Question 6

All of the following are important in evaluating a positive DAT except:

Answer 6

donation history

Question 7

Saline used for blood banking tests should have a pH of_______.

Answer 7

7.2 to 7.4

Question 8

Anti-IgG is specific for what part of the IgG molecule?

Answer 8

Fc fragment

Question 9

An advantage of monoclonal anti-C3 over polyclonal anti-C3 is:

Answer 9

The antibody potency can be controlled

Question 10

What is the action of PEG?

Answer 10

Removes water molecules thereby concentrating antibodies