Øvelse I cellen og dens komponenter

Question 1

Answer 1

Restriction enzyme, mRNA, reverset ranscriptase

Question 2

A PCR reaction consists of 3 steps

Answer 2

DNA denaturatoin, primer annealing and elongation

Question 3

A PCR reaction that continues for 30 cycles will produce approximately ? PCR products from a single template DNA molecule?

Answer 3

1.000.000.000

Question 4

Answer 4

1+3

Question 5

Answer 5

Question 6

We use these enzymes in lab exercise I to cleave genomic DNA from porcine small intestine before electrophoresis.

Which of the following ansers is/are true?

Restriction enzymes cleave DNA sequences randomly

Restriction enzymes are exonucleases

Restriction enzymes are endonucleases

Restriction enzymes recognize and cleave specific sequences about 4-8 base pairs long

Answer 6

Restriction enzymes are endonucleases

Restriction enzymes recognize and cleave specific sequences about 4-8 base pairs long

Question 7

Which of the following anwers is/are true?

MNase cleaves DNA sequences randomly

MNase recognizes and cleaves specific sequences about 4-8 base pairs long

MNase is a endonuclease

MNase is a protease

Answer 7

MNase is a endonuclease

Question 8

Answer 8

2

Question 9

Which of the statements about gel electrophoresis is/are true?

Answer 9

Question 10

Which of the following statements about SDS-PAGE is/are true?

Answer 10

Question 11

Answer 11

1+2

Question 12

DNA purification

Answer 12

Question 13

RNA purification

Answer 13

Question 14

GEL-ELECTROPHORESIS OF DNA AND RNA

Answer 14

Question 15

Microcroccus nucelase treatment of chromatin

Answer 15

Question 16

Restriction enzymes

Answer 16

Question 17

PCR

Answer 17

Question 18

RT-PCR

Answer 18

Question 19

Qualitative determination of proteins

Answer 19