Organelles & compartmentalisation - cell organelles Flashcards

1
Q

What type of cells have a more complex ultrastructure, eukaryotic or prokaryotic?

A

Eukaryotic cells

Eukaryotic cells have membrane-bound organelles which contribute to their complexity.

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2
Q

What are the membrane-bound compartments in eukaryotic cells called?

A

Organelles

Organelles can be bound by a single or double membrane.

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3
Q

Which structures are not considered organelles due to the absence of a membrane?

A
  • Cell wall
  • Cytoskeleton
  • Cytoplasm

These structures lack the membrane-bound characteristic of organelles.

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4
Q

Name four organelles found in eukaryotic cells.

A
  • Nucleus
  • Vesicles
  • Ribosomes
  • Plasma membrane

Each organelle has specific functions within the cell.

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5
Q

What is one advantage of compartmentalisation in cells?

A
  • Localisation of enzymes and substrates at higher concentrations
  • Separation of damaging substances
  • Maintenance of optimal conditions for processes
  • Alteration of organelle numbers and locations based on cell requirements

Compartmentalisation increases efficiency and safety in cellular processes.

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6
Q

How do organelles become specialised for specific functions?

A

Through specific adaptations

The structure of each organelle is adapted to help it carry out its particular function.

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7
Q

Why is the separation of organelles from the rest of the cell important?

A

It allows organelles to carry out their own chemical reactions without interference

This separation is facilitated by membranes, which can be single or double.

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8
Q

What scientific advancement has allowed the study of individual organelles?

A

The invention of ultracentrifuges

Ultracentrifuges enable cell fractionation, which is crucial for studying organelles.

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9
Q

What is the process called that allows separation of cell components for study?

A

Cell fractionation

This process involves breaking up tissue samples and centrifuging them.

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10
Q

What are the three stages of cell fractionation?

A
  • Homogenisation
  • Filtration
  • Ultracentrifugation

Each stage serves a specific purpose in isolating organelles.

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11
Q

What is the purpose of homogenisation in cell fractionation?

A

To break up the cell sample

This is typically done using a homogeniser, similar to a blender.

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12
Q

What is performed during the filtration stage of cell fractionation?

A

The homogenate is filtered through gauze

This step removes debris and larger particles from the mixture.

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13
Q

What does a centrifuge do?

A

Separates materials by spinning

The speed can be adjusted to separate components based on molecular weight.

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14
Q

True or False: Research into separate organelles was extensive before the invention of ultracentrifuges.

A

False

Prior to ultracentrifuges, research opportunities were limited.

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