OB D7 - Amelogenesis Flashcards
what is enamel?
mineralised epithelial product
what is the composition of enamel?
-96% -hydroxyaptite
-1-2% organic matrix :
enamel proteins
-2% - water
what is not found in enamel?
collagen
when does enamel form?
after dentine mineralises
what happens in amelogenesis?
- pre-ameloblast (polarised nucleus and lots of organelles of synthesis and secretion
- basal lamina disintegrates
- now ameloblasts
- enamel formation begins
- cells retreat outwards and towards incised aspect (upwards and out)
- ameloblasts changes shape and a point forms a point (tomes process)
what is the point on an ameloblast called?
Tomes process
what is the final cue for onset of amelogenesis?
- dentine formation
- breakdown of basal lamina
what does an ameloblast secrete?
organic matrix
what occurs immediately after organic matrix is secreted?
partial mineralisation
how does maturation occur in amelogenesis?
- influx of mineral ions
- breakdwon and removal of most of the organic matrix
what enamel proteins are in the amorphous gel of the organic matrix (enamel)?
- amelogenins (90%)
- enamelins - (~2%) strongly binds to mineral
- tuftelin - confined to ADJ
In addition to enamel proteins, what else is found in amorphous gel?
enzymes
Describe properties of amelogenins.
- Rich in proline and glutamine
- Similar in different species (clinically useful)
- Hydrophobic (aggregate)
- Spread throughout the developing enamel thickness
- Thixotropic.
what is the role of enamel proteins?
- aid of nucleation of hydroxyapatite (epitactic matrix)
- orientate and stabilise crystal growth
- broken down and let during maturation
what is not involved in enamel mineralisation?
- No “pre-enamel”
- No MVs
Describe the changes in composition of enamel during mineralisation from initial to rapid to maturation.
Initial secretion : -virtualy immediate mineralisation -15% mineral, 65% water and 20% proteins Rapid mineralisation to 30%: -immature or young enamel Maturation : -further mineralisation to 96%
What happens during maturation and what does it require?
-thin crystals grow in thickness
-requires:
mineral ions IN and enamel matrix OUT
what happens to amelogenin (enamel protein) in maturation?
– flows under pressure (Thixotropic)
– enzymes break down amelogenins
– bulk of breakdown products removed
what is the matrix that flows out?
amelogenins
what is the matrix that remains?
enamelin
what happens once most of enamel thickness is laid down?
Tomes process is lost
what does the loss of tomes process result in?
last formed enamel is aprismatic
what else is also aprismatic?
1st formed 5μm (no tomes process
what is formed when maturation is complete?
enamel cuticle ( final ameloblast secretion) -1μm thick - like a basal lamina
what forms when enamel formation is complete?
reduced enamel epithelium
what is reduced enamel epithelium derived from?
- reduced ameloblasts
- other remnants of the enamel organ OEE etc
what is reduced enamel epithelium attached to?
cuticle
what are the 3 functions of reduced enamel epithelium function?
1. Protection of enamel surface from: • resorption • prevention of cementum formation 2. Provide an epithelial lined pathway for eruption 3. Forms initial junctional epithelium
what is the fate of the reduced enamel epithelium?
- fusion of basal lamina
- cell death
- forms junction epithelium