Nucleotide synthesis

Question 1

Methotrexate

Answer 1

Folic acid analog. Cancer therapy. Inhibits Purine synthesis. inhibits H2Folate to H4Folate. H4Folate is cofactor in purine synth

Question 2

Mycophenolic acid

Answer 2

depletes T and B cell purines. inhibits IMP dehydrogenase.

immunosuppresant. prevent graft rejections

Question 3

ADA def

Answer 3

dATP buildup.
dATP inhibits DNA synthesis.
T and B cell depletion–>infection
SCID (Severe Combined Immunodeficiency Disease)

Question 4

Gout

Answer 4

hyperuricemia. deposition of uric acid/urate crystals in joints (NOT UREA!) arthritic joint inflammation. underexcretion of uric acid.

anti-inflammatory drugs
Allopurinol: lowers uric acid synthesis

Question 5

PNP def

Answer 5

ONLY T cell depleted. B cells not affected

–>infection

Question 6

Lesch Nyhan

Answer 6

necrotizing encephalopathy. gout-like symptoms
X-linked, heritable
increased PRPP levels
increased hyypoxanthine, guanine

Allopurinol: lowers uric acid synthesis

Question 7

Orotic Aciduria

Answer 7

pyrimidine synth def.
Orotic Acid in urine

treated with Uridine. inhibits CPS II

Question 8

chronic Myelogenous Leukemia (CML)

Answer 8

cancer

Hydroxyurea: inhibits generation of dNTs.
also treats Sickle Cell

Question 9

5FU

Answer 9

Thymine analog. antitumor agent

Question 10

Trimethoprim

Answer 10

treats UTIs

Question 11

Folate def

Answer 11

malnutrition (ise Alcoholics). megaloblastic/macrocytic anemia.

pregnancy Folate deficiency–>spinal defects

increase green veg, citrus, folic acid supplements.