Nucleotide Metabolism Flashcards
Roth
Acyclovir wreks the viral version of _____ Kinase
Thymidine
The Precursor to Thymidine Monophosphate (TMP) is deoxy_____
UMP
Ribavirin and Mycophenolic Acid inhibit _____, and therefore deplete guanine nucleotides
IMP dehydrogenase
Azaserine, 6-Diazo-5-Oxonorleucine (DON) are analogs of Glutamine and are potent irreversible inhibitors to the enzyme _____, which is the committed step of Purine Synthesis
PRPP-amido-transferase
Since Allopurinol can be scavenged it can decrease the levels of _____ and can also inhibit _____ amidotransferase to further wreck de novo purine synthesis
PRPP, PRPP
In Purine De novo synth, ____ is the activator and the Di-Tri “high energy” Purines are the inhibitors of PRPP Synthetase
Phosphate
Lesch-Nyhan syndrome is x-linked recessive with a deficiency in the enzyme _____, needed for Guanine salvaging
Guanine phosphoribosyl-transferase (HGPRT)
Pyrimidine bases are degraded to _____ products
Soluble
Orotic Aciduria can be treated with ____, ____, or _____ which would all be formed after the Orotate step
CMP, UMP, Uridine
The location of CPSII, in Purine synthesis is located in the _____ whereas CPS I for Urea cycle is found in the ____
Cytoplasm, Mitochondria
Xanthine Oxidase works on both _____ and _____ as well as the medication ____
Hypoxanthine, Xanthine, Allopurinol
In Pyrimidine Synth, Carbamoyl Phosphate Synthetase II is Inhibited by UTP and activated by ATP and _____, which makes sense because if you’re making Purines, then you want to crank out pyrimidine synthesis as well.
PRPP
When Thioredoxin is reduced, Ribonucleotide reductase replaces the OH group on the Ribonucleoside diphosphate (RDP) with an H that came from _________
NADPH
In addition to Adenosine Deaminase (ADA) deficiency, _____ deficiency causes T-cell problems as well
Purine Nucleoside Phosphorylase (PNP)
When the Salvage pathways are blocked, the low energy purines AMP, IMP, and GMP, levels go _____, which leaves the body strictly dependent on PRPP de novo Crank-up Boost resulting in lots of Hypoxanthine, Xanthine, and Uric Acid to cause _____
down, Gout
Methotrexate inhibits both purine and ____ synthase synthesis
Thymidylate
____ Deaminase deficiency causes SCID, involving T-cell and B-cell dysfunction, due to the buildup of _____, which ultimately inhibits Ribonucleotide Reductase
Adenosine (ADA), dATP
Purine degradation causes a buildup of _____ _____
Uric Acid
Fluorouracil creates FdUMP which binds to the enzyme _____
Thymidylate Synthase
In the committed step of Purine Synth PRPP Amido-transferase gets activated by molecules “Low-energy” purines such as _____, _____, and _____.
AMP, GMP, IMP
Methotrexate and Aminopterin Inhibits ______ which inhibits the creation of THF from Folate, and thus blocks Purine and Pyrimidine synthesis
Dihydrofolate Reductase (DHFR)
In Pyrimidine Synthesis, A defect in OMP Decaboxylase or Transferase will result in a buildup of Orotate and put you at risk for _____ _____
Orotic Aciduria