Nucleotide Metabolism

Question 1

Acyclovir wreks the viral version of _____ Kinase

Answer 1

Thymidine

Question 2

The Precursor to Thymidine Monophosphate (TMP) is deoxy_____

Answer 2

UMP

Question 3

Ribavirin and Mycophenolic Acid inhibit _____, and therefore deplete guanine nucleotides

Answer 3

IMP dehydrogenase

Question 4

Azaserine, 6-Diazo-5-Oxonorleucine (DON) are analogs of Glutamine and are potent irreversible inhibitors to the enzyme _____, which is the committed step of Purine Synthesis

Answer 4

PRPP-amido-transferase

Question 5

Since Allopurinol can be scavenged it can decrease the levels of _____ and can also inhibit _____ amidotransferase to further wreck de novo purine synthesis

Answer 5

PRPP, PRPP

Question 6

In Purine De novo synth, ____ is the activator and the Di-Tri “high energy” Purines are the inhibitors of PRPP Synthetase

Answer 6

Phosphate

Question 7

Lesch-Nyhan syndrome is x-linked recessive with a deficiency in the enzyme _____, needed for Guanine salvaging

Answer 7

Guanine phosphoribosyl-transferase (HGPRT)

Question 8

Pyrimidine bases are degraded to _____ products

Answer 8

Soluble

Question 9

Orotic Aciduria can be treated with ____, ____, or _____ which would all be formed after the Orotate step

Answer 9

CMP, UMP, Uridine

Question 10

The location of CPSII, in Purine synthesis is located in the _____ whereas CPS I for Urea cycle is found in the ____

Answer 10

Cytoplasm, Mitochondria

Question 11

Xanthine Oxidase works on both _____ and _____ as well as the medication ____

Answer 11

Hypoxanthine, Xanthine, Allopurinol

Question 12

In Pyrimidine Synth, Carbamoyl Phosphate Synthetase II is Inhibited by UTP and activated by ATP and _____, which makes sense because if you’re making Purines, then you want to crank out pyrimidine synthesis as well.

Answer 12

PRPP

Question 13

When Thioredoxin is reduced, Ribonucleotide reductase replaces the OH group on the Ribonucleoside diphosphate (RDP) with an H that came from _________

Answer 13

NADPH

Question 14

In addition to Adenosine Deaminase (ADA) deficiency, _____ deficiency causes T-cell problems as well

Answer 14

Purine Nucleoside Phosphorylase (PNP)

Question 15

When the Salvage pathways are blocked, the low energy purines AMP, IMP, and GMP, levels go _____, which leaves the body strictly dependent on PRPP de novo Crank-up Boost resulting in lots of Hypoxanthine, Xanthine, and Uric Acid to cause _____

Answer 15

down, Gout

Question 16

Methotrexate inhibits both purine and ____ synthase synthesis

Answer 16

Thymidylate

Question 17

____ Deaminase deficiency causes SCID, involving T-cell and B-cell dysfunction, due to the buildup of _____, which ultimately inhibits Ribonucleotide Reductase

Answer 17

Adenosine (ADA), dATP

Question 18

Purine degradation causes a buildup of _____ _____

Answer 18

Uric Acid

Question 19

Fluorouracil creates FdUMP which binds to the enzyme _____

Answer 19

Thymidylate Synthase

Question 20

In the committed step of Purine Synth PRPP Amido-transferase gets activated by molecules “Low-energy” purines such as _____, _____, and _____.

Answer 20

AMP, GMP, IMP

Question 21

Methotrexate and Aminopterin Inhibits ______ which inhibits the creation of THF from Folate, and thus blocks Purine and Pyrimidine synthesis

Answer 21

Dihydrofolate Reductase (DHFR)

Question 22

In Pyrimidine Synthesis, A defect in OMP Decaboxylase or Transferase will result in a buildup of Orotate and put you at risk for _____ _____

Answer 22

Orotic Aciduria