Nucleic Acids - Structure and Function 8 Flashcards
For DNA, compression comes at different levels. The first level is at ___________
nucleosomal level
are stacked and coiled into higher order structures
Nucleosomes
simplest higher order structures
10 nm fibers
consist of stacked nucleosomes and they are packed tightly
30 nm fibers
Higher level packing produces the ____________ found in meiosis and mitosis
metaphase chromosome
logistical concern for the process of DNA replication and gene expression where specific regions of DNA must be transcribed
chromatin complex
True or False: Altering chromatin structure is therefore an essential function for transcriptional activation in prokaryotes
False; eukaryotes
True or False: In transcriptional activation, one strategy involves adding acetyl groups to the positively charged lysine side chains to loosen their grip on the negatively charged DNA, thus allowing lesser access of proteins involved in activating transcription to gain access to the DNA
False; greater access
an analytical method that allows to determine whether a compound causes mutations in DNA or not
Ames test
Ames test is invented by
Dr. Bruce Ames
single base pair of a selectable marker of an organisms is mutated in a plasmid to render it non-functional
Ames test
True or False: In ames test, the absence of mutation means that no bacteria will grow. The more colonies of bacteria that grow, the more mutation happened
True
True or False: In ames test, the absence of mutation means that more bacteria will grow. The less colonies of bacteria that grow, the more mutation happened
False;The mutation means that more colonies will grow
The Ames test identifies about _____ of known mutagens
90%
