NONTUBERCULOSIS MYCOBACTERIA Flashcards
- Slowly Growing Species
NONTUBERCULOUS MYCOBACTERIA
part of the Mycobacterium avium complex (MAC).
- Mycobacterium avium and M. intracellulare
o Also includes M. avium subsp. paratuberculosis and M. avium subsp. silvaticum
part of the Mycobacterium avium complex (MAC)
- environmental saprophytes and have been recovered from soil, water, house dust, and other environmental sources
MYCOBACTERIUM AVIUM COMPLEX
a cause of disease in poultry and swine
MYCOBACTERIUM AVIUM COMPLEX
- Zoonotic microorganisms
MYCOBACTERIUM AVIUM COMPLEX
- the cells are short, coccobacillary, and uniformly stained, without beading or banding
MYCOBACTERIUM AVIUM COMPLEX
- production of a heat-stable catalase and the ability to grow on media containing 2μg/ mL of T2H
MYCOBACTERIUM AVIUM COMPLEX
- Causative agent of Johne disease, an intestinal infection occurring as a chronic diarrhea in cattle, sheep, goats, and other ruminants
MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS
- very slow growth rate (3 to 4 months
MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS
- Needs mycobactin-supplemented medium for primary isolation.
MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS
- cause of disseminated infections in patients with AIDS * enteritis and genital and soft tissue infections
- Middlebrook 7H11 agar supplemented with mycobactin
- heat-stable catalase, pyrazinamidase, and urease
- Submandibular lymphadenitis, subcutaneous nodules, painful swellings, ulcers progressing to abscesses, and draining fistulas are often the clinical manifestations
- A unique characteristic of this organism is its requirement for hemoglobin or hemin for growth.
- Chocolate (CHOC) agar, Mueller-Hinton agar with 5% Fildes enrichment, and Löwenstein- Jensen (LJ) medium containing 2% ferric ammonium citrate.
- Optimal growth temperature is 28° C to 32° C; little or no growth occurs at 37° C. cells are strongly acid-fast, short, occasionally curved bacilli without banding or beading, and arranged in tight clusters or cords
under group III
strains have been isolated from
water
- Infections are not normally considered contagious from person to person
- Susceptible to rifampin and ethambutol
partially resistant to isoniazid and streptomycin
resistant to pyrazinamide
- A multidrug regimen of isoniazid, rifampin, and ethambutol is
currently recommended
- Long rods with distinct crossbanding
- Colonies appear smooth to rough, with characteristic wavy edges and dark centers when grown on Middlebrook 7H10 agar
Colonies are photochromogenic
- With prolonged exposure to light, most strains form dark red crystals of B-carotene on the surface of and inside the colony
- strongly catalase-positive
- hydrolyze Tween 80 in 3 days
*
strong nitrate reduction
- Pyrazinamidase production
- Under Group I
- Chronic pulmonary disease and cervical lymphadenitis
- Resistant to isoniazid, streptomycin, p- aminosalicylic acid, and rifampin
susceptible to ethambutol and cycloserine.
- Short coccobacillus without cross bands on acid- fast–stained smears.
- Colonies are smooth glistening, and opaque, with dense centers
- Cervical lymphadenitis in children
- Group II
MYCOBACTERIUM SCROFULACEUM
- Isolated from the lymph nodes of monkeys
MYCOBACTERIUM SIMIAE
- Colonies on Middlebrook 7H10 agar are thin, transparent or tiny, and filamentous
- Group I
MYCOBACTERIUM SIMIAE
rare cause of mycobacteriosis, also referred to as Buruli ulcer
- Acid-fast cells are long, without beading or crossbanding
- Group III
has been implicated in
diseases of fish and isolated from aquariums
MYCOBACTERIUM MARINUM
- Cutaneous infections in humans occur when traumatized skin comes into contact with salt water or inadequately chlorinated fresh water containing the organism.
MYCOBACTERIUM MARINUM
- Susceptible to rifampin and ethambutol
MYCOBACTERIUM MARINUM
resistant to isoniazid and pyrazinamide
MYCOBACTERIUM MARINUM
partially resistant or intermediate to streptomycin
MYCOBACTERIUM MARINUM
are moderately long to long rods with cross barring.
MYCOBACTERIUM MARINUM CELLS
- Colonies grown or exposed to light develop a deep yellow color
MYCOBACTERIUM MARINUM CELLS
- Growth is optimum at incubation temperatures of
28° C to 32° C
- None reduces nitrate or produces heat-stable catalase
MYCOBACTERIUM MARINUM CELLS
- The organisms hydrolyze Tween 80 and produce urease and pyrazinamidase.
- Group I
MYCOBACTERIUM MARINUM CELLS
- Recovered from hot and cold water taps (including water storage tanks of hospitals) and birds (hotspings)
MYCOBACTERIUM XENOPI
- Susceptible to the quinolones (ciprofloxacin, ofloxacin);
MYCOBACTERIUM XENOPI
some isolates are susceptible to vancomycin, erythromycin, or cefuroxime
MYCOBACTERIUM XENOPI
- Colonies on Middlebrook 7H10 agar are small, with dense centers and filamentous edges.
MYCOBACTERIUM XENOPI
reveals distinctive round colonies with branching and filamentous extensions; aerial hyphae are usually seen in rough colonies.
- Cornmeal-glycerol agar
- Young colonies grown on cornmeal agar have a bird’s nest appearance, with characteristic sticklike projections.
- Group II
MYCOBACTERIUM XENOPI