MYCOBACTERIUM TUBERCULOSIS COMPLEX Flashcards
MYCOBACTERIUM TUBERCULOSIS COMPLEX
- Mycobacterium tuberculosis (Koch’s bacillus)
- Mycobacterium bovis
- Mycobacterium africanum
- Mycobacterium canetti
- Mycobacterium microti
- Longest replication time
MYCOBACTERIUM TUBERCULOSIS
Cord factor
VF of MYCOBACTERIUM TUBERCULOSIS
slow-growing; buff color; raised and dry; “CAULIFLOWER-LIKE APPEARANCE”. Rough colonies exhibit “cording” (Curve strands bacilli)
culture of MYCOBACTERIUM TUBERCULOSIS
(+) Niacin and Nitrate reduction
BT of MYCOBACTERIUM TUBERCULOSIS
20 to 22 hrs
replication time for m tuberculosis
- Tuberculosis in human and animals (cattle, dogs, cat and swine)
MYCOBACTERIUM BOVIS
- Attenuated strain used for vaccination (Bacillus- Calmette-Guerin or BCG vaccine)
MYCOBACTERIUM BOVIS
: Slow-growing, small, granular, rounded and non-pigmented
Culture of MYCOBACTERIUM BOVIS
(-) Niacin and Nitrate reduction
BT of MYCOBACTERIUM BOVIS
- Associated human cases of tuberculosis in tropical Africa
MYCOBACTERIUM AFRICANUM
- Detection of organism requires the use of spoligotyping (spacer oligotyping
MYCOBACTERIUM AFRICANUM
- Smooth strain of M. tuberculosis
MYCOBACTERIUM CANETTI
- Grows more rapidly than M. tuberculosis (6 days)
MYCOBACTERIUM CANETTI
- Isolated from an AIDS patient with mesenteric tuberculosis
MYCOBACTERIUM CANETTI
(+) Niacin and Nitrate reduction
BT of MYCOBACTERIUM CANETTI
Isolated from TB patients both immunocompromised and immunocompetent individuals
MYCOBACTERIUM MICROTI
M. avium, M. intracellulare, M. avium subsp. Paratuberculosis and M. avium subsp. silvaticum (wood pigeon bacillus)
MYCOBACTERIUM AVIUMCOMPLEX Specie
- Most common cause of Pulmonary infection to human, pathogen in AIDS.
MYCOBACTERIUM AVIUM COMPLEX
most common site of colonization and dissemination in AIDS.
GI tract
Pleomorphic, short coccobacilli without beading;
MYCOBACTERIUM AVIUM COMPLEX
(+) PAS
(+) Heat stable catalase
BT of MYCOBACTERIUM AVIUM COMPLEX
Second to M. avium complex to cause NTM lung disease (Chronic cavitary pulmonary lesion)
MYCOBACTERIUM KANSASII (YELLOW BACILLUS)
- Not contagious from person to person
MYCOBACTERIUM KANSASII (YELLOW BACILLUS)
Long rods with distinct crossbanding
microscopy of MYCOBACTERIUM KANSASII (YELLOW BACILLUS)
Dark red crystals of 10-B- carotene
photochromogens of MYCOBACTERIUM KANSASII (YELLOW BACILLUS)
MB7H10: smooth to rough with dark centers and waxy edges
culture of of MYCOBACTERIUM KANSASII (YELLOW BACILLUS)
- Disease of fishes and isolated from aquariums
MYCOBACTERIUM MARINUM
Swimming pool granuloma” red or bluish red nodule on the elbow, knee, toe or finger. Occurs when an open wound comes in contact with contaminated chlorinated fresh water or salt water
Causative agent of MYCOBACTERIUM MARINUM
Fresh water and salt water
natural reservoir of MYCOBACTERIUM MARINUM
: Long rods with cross barring
microscopy ofMYCOBACTERIUM MARINUM
- : smooth to tough, wrinkled, yellow
- Culture of MYCOBACTERIUM MARINUM
- Third most common Mycobacterium species after M. tuberculosis and M. leprae
MYCOBACTERIUM ULCERANS
: Buruli ulcer (painless nodule under the skin after previous trauma)
Cause of MYCOBACTERIUM ULCERANS
Moderately long rods without cross- banding
MYCOBACTERIUM ULCERANS
smooth, rough and non-pigmented (6- 12 weeks incubation
MYCOBACTERIUM ULCERANS
: (+) Heat stable catalase
BT of MYCOBACTERIUM ULCERANS
- Contaminates the tap water used by the patients in rinsing their mouths prior to the procedure for sputum collection.
MYCOBACTERIUM GORDONAE (TAP WATER BACILLUS)
- Rarely cause infection to human
MYCOBACTERIUM GORDONAE (TAP WATER BACILLUS)
Smooth and yellowish-orange colored
MYCOBACTERIUM GORDONAE (TAP WATER BACILLUS)
(+) Tween 80 hydrolysis and heat- stable catalase, (-) nitrate reduction
BT of MYCOBACTERIUM GORDONAE (TAP WATER BACILLUS)
- Recovered from hot and cold- water taps, hospital storage tanks
Mycobacterium xenopi
- Potential pathogen of pulmonary infection in adults
MYCOBACTERIUM XENOPI
- Non-photochromogenic and scotochromogenic
MYCOBACTERIUM XENOPI
classified with non- photochromogenic group however, colonies are frequently bright yellow.
MYCOBACTERIUM XENOPI
Long and filamentous
microscopy of MYCOBACTERIUM XENOPI
MB7H10: small with filamentous edges
* Cornmeal glycerol agar: branching filaments
culture of MYCOBACTERIUM XENOPI
- Growth: 42°C
growth of MYCOBACTERIUM XENOPI
(+) heat stable catalase, pyrazinamidase
BT of MYCOBACTERIUM XENOPI
- Once incubated in absence of light, they will be
scrotochromogens with yellow pigment at 42°C
- Normally saprophytic and rarely causes human infections
MYCOBACTERIUM TERRAE COMPLEX
M. terrae, M. triviale and M. nonchromogenicum
specie of MYCOBACTERIUM TERRAE COMPLEX
Short to medium coccobacilli
microscopy of MYCOBACTERIUM TERRAE COMPLEX
rough and dry
culture of M. triviale
smooth
culture of M. terrae:
smooth to rough and white to buff
culture of M. nonchromogenicum:
(+) Tween 80 hydrolysis and heat stable catalase, (+) growth in 5% NaCl (M. terrae
Biochemical test of YCOBACTERIUM TERRAE COMPLEX