Non-Fermentative Gram (-) Bacilli

Question 1

causes Shanghai fever (mild form of typhoid)

Necrotizing pneumonia (fleur-de-lis pattern)

Ecthyma gangrenosum

Answer 1

P. aeruginosa

Question 2

greenish metallic colonies on blood agar

Answer 2

P. aeruginosa

Question 3

Nosocomial organisms similar to Pseudomonas:

Answer 3

P. aeruginosa

Question 4

It is the most common pathogen isolated from patients who have been hospitalized longer than 1 week, and it is a frequent cause of nosocomial infections.

Answer 4

P. aeruginosa

Question 5

PSEUDOMONAS

Answer 5

-Pneumonia, pyocyanin
-Sepsis
-Ecthyma gangrenosum
-UTIs
-Diabetes, drug use
-Osteomyelitis (eg, puncture wounds)
-Mucoid polysaccharide capsule
-Otitis externa (swimmer’s ear) —–Nosocomial infections (catheters, equipment)
Exotoxin A
Skin infections (hot tub folliculitis)

Question 6

with sweet, fruity grape-like odor

› produces pigments:
- Pyocyanin (blue)
- Pyoverdin (green, fluorescent

Answer 6

P. aeruginosa

Question 7

-agent of blue pus
-grape like or corn like odor

Answer 7

P. aeruginosa

Question 7

test to differentiate enterobacteriaceae form pseudomonas

Hint: uses Hugh and Leifson medium

Answer 7

oxidative-fermentative test (OF) test

It determines the ability of organisms to utilize a substrate thereby producing acid by products in the presence or absence of oxygen

open tube (+) yellow

Question 8

-found in hot tubes and whirlpool baths
-ecthyma gangrenosum
-swimmer’s ear
-jacuzzi syndrome
-etiologic agent of lung infection in cystic fibrosis pxs
-nosocomial related to antibiotic resistance

Answer 8

P. aeruginosa

Question 9

-grow at 42c
-acetamide/ citrate +
-GLUCONATE PRODUCTION
-ARGININE DIHYDROLASE

Answer 9

P. aeruginosa

Question 10

Spp. isolated from contaminated blood products, cosmetics, hospital equipments, urine, and respiratory specimens

Answer 10

Pseudomanas flourecens
Pseudomonas putida

Question 11

differential test for

Pseudomanas flourecens
Pseudomonas putida

Answer 11

(+) gelatin hydrolysis for P. fluorescens

Question 12

medium that promotes pigment production of Pseudomanas flourecens
Pseudomonas putida

Answer 12

Seller’s medium

Question 13

can be mistaken for Neisseria spp under the microscope

Answer 13

Acinetobacter

Question 14

3rd most common isolated non-fermen gram neg baccilli

-motile at 42C

-affinity to MALTOSE and not glucose

Answer 14

Stenorophomonas maltophilia

Question 15

BAP - lavender-green to light purple
MAC - BLUE color

Answer 15

Stenorophomonas maltophilia

Question 16

MAC colonies: Purple color
BAP: non-wrinkled yellow or yellow-green color

Answer 16

Acinetobacter

Question 17

isolated from Anesthetics
-grows well on most culture media but may lose its viability on the former in three to four days

Answer 17

Burkholderia cepacia

Question 18

agent of glanders or farcry disease affects horses and donkeys

Answer 18

Burkholderia mallei

Question 19

AKA Vietnamese time bomb
agent of MELIOIDOIS

-Culture: Ashdown medium with colistin
colonies are dry, wrinkled with DEEP PINK COLOR

Answer 19

Burkholderia pseudomallei

Question 20

Apple/strawberry/fruity odor

Answer 20

Alcaligenes faecalis

Question 21

agent of blepharoconjunctivitis and and angular conjunctivitis

Answer 21

Moraxella lacunata

Question 22

non motile member of burkholderia

Answer 22

Burkholderia mallei

Question 23

pigment of P. aeruginosa not found in other species of Pseudomonas?

Answer 23

Pyocyanin

Question 24

Pseudomas spp found contaminated blood products

Answer 24

Pseudomonas putida