Non-Fermentative Gram (-) Bacilli Flashcards
causes Shanghai fever (mild form of typhoid)
Necrotizing pneumonia (fleur-de-lis pattern)
Ecthyma gangrenosum
P. aeruginosa
greenish metallic colonies on blood agar
P. aeruginosa
Nosocomial organisms similar to Pseudomonas:
P. aeruginosa
It is the most common pathogen isolated from patients who have been hospitalized longer than 1 week, and it is a frequent cause of nosocomial infections.
P. aeruginosa
PSEUDOMONAS
-Pneumonia, pyocyanin
-Sepsis
-Ecthyma gangrenosum
-UTIs
-Diabetes, drug use
-Osteomyelitis (eg, puncture wounds)
-Mucoid polysaccharide capsule
-Otitis externa (swimmer’s ear) —–Nosocomial infections (catheters, equipment)
Exotoxin A
Skin infections (hot tub folliculitis)
with sweet, fruity grape-like odor
› produces pigments:
- Pyocyanin (blue)
- Pyoverdin (green, fluorescent
P. aeruginosa
-agent of blue pus
-grape like or corn like odor
P. aeruginosa
test to differentiate enterobacteriaceae form pseudomonas
Hint: uses Hugh and Leifson medium
oxidative-fermentative test (OF) test
It determines the ability of organisms to utilize a substrate thereby producing acid by products in the presence or absence of oxygen
open tube (+) yellow
-found in hot tubes and whirlpool baths
-ecthyma gangrenosum
-swimmer’s ear
-jacuzzi syndrome
-etiologic agent of lung infection in cystic fibrosis pxs
-nosocomial related to antibiotic resistance
P. aeruginosa
-grow at 42c
-acetamide/ citrate +
-GLUCONATE PRODUCTION
-ARGININE DIHYDROLASE
P. aeruginosa
Spp. isolated from contaminated blood products, cosmetics, hospital equipments, urine, and respiratory specimens
Pseudomanas flourecens
Pseudomonas putida
differential test for
Pseudomanas flourecens
Pseudomonas putida
(+) gelatin hydrolysis for P. fluorescens
medium that promotes pigment production of Pseudomanas flourecens
Pseudomonas putida
Seller’s medium
can be mistaken for Neisseria spp under the microscope
Acinetobacter
3rd most common isolated non-fermen gram neg baccilli
-motile at 42C
-affinity to MALTOSE and not glucose
Stenorophomonas maltophilia
BAP - lavender-green to light purple
MAC - BLUE color
Stenorophomonas maltophilia
MAC colonies: Purple color
BAP: non-wrinkled yellow or yellow-green color
Acinetobacter
isolated from Anesthetics
-grows well on most culture media but may lose its viability on the former in three to four days
Burkholderia cepacia
agent of glanders or farcry disease affects horses and donkeys
Burkholderia mallei
AKA Vietnamese time bomb
agent of MELIOIDOIS
-Culture: Ashdown medium with colistin
colonies are dry, wrinkled with DEEP PINK COLOR
Burkholderia pseudomallei
Apple/strawberry/fruity odor
Alcaligenes faecalis
agent of blepharoconjunctivitis and and angular conjunctivitis
Moraxella lacunata
non motile member of burkholderia
Burkholderia mallei
pigment of P. aeruginosa not found in other species of Pseudomonas?
Pyocyanin
Pseudomas spp found contaminated blood products
Pseudomonas putida
