Culture Media Preparation🧫

Question 1

Amount of agar in solid media

Answer 1

2-3%

Question 2

Amount of agar in semi-solid media

Answer 2

0.5-1%

Question 3

According to chemical composition

All ingredients are known

Answer 3

Synthetic media

e.g. Ringers solution, Locke’s solution

Question 4

According to chemical composition

All ingredients are unknown

Answer 4

Non-synthetic media

e.g. meat extract broth

Question 5

According to their use/functional type

Not enriched for non-fastidious

Answer 5

Simple media

e.g. NA, Nutrient broth

Question 5

According to their use/functional type

for cultivation of a specific/particular organism

Answer 5

Specific media

Question 6

According to their use/functional type

includes 5% sheep blood agar, and several additives

Answer 6

Enriched media

Question 6

examples of a transport media

Answer 6

Cary blair medium, amies transport medium

Question 7

Steps in preparation of Plate media

Answer 7

1. weigh
2. autoclave
3. dispense

except: SSA, TCBS

Question 8

Steps in preparation of tube media

Answer 8

1. weigh
2. dispense
3. autoclave

Question 9

Thioglycollate broth

Answer 9

for cultivation and isolation of obligate and facul. anaerobic microaerophilic bacteria

Question 9

NOTE: SSA medium

Answer 9

means Shigella, Salmonella agar medium

Question 10

SIM

Answer 10

differentiates members of enterobactericeae

Question 10

NA broth

Answer 10

for non and fastidous

Question 10

TSI

Answer 10

differentiates based on their fermentation of:

LACTOSE
GLUCOSE
SUCROSE

Question 11

SCA
(starch casein agar)

Answer 11

detection of saccharide marine bacteria and mostly actinomycetes

Question 12

frequently used to differentiate among various Gram-negative bacilli that are isolated from fecal specimens. (Enterecs)

Answer 12

MacConkey agar

(pink colonies=ferments lactose)

Question 13

a selective and differential medium, is used to screen for Staphylococcus aureus. HAS 7.5% SODIUM CHLORIDE concentration

Answer 13

MSA

(yellow colonies = s. aureus)