Neutrophil Killing + Macrophages

Question 1

Reactions that form reactive oxygen species in acute inflammation

Answer 1

O2 –> O2- via NADPH oxidase
O2- –> H2O2 via superoxide dysmutase
H202 –> HOCl via MPO

Question 2

Two potential pathologies of ROS generation

Answer 2

NADPH oxidase deficiency: O2 –> O2-

MPO deficiency: H2O2 –> HOCl

Question 3

NADPH deficiency
defect
complications
Dx

Answer 3

NADPH oxidase defect: O2 –> O2-
oxidase positive bug infections (esp Pseudomonas cepacia)
+ nitroblue tetrazolium test

Question 4

MPO deficiency
defect
complications
Dx

Answer 4

MPO defect: H2O2 –> HOCl
recurrent Candida infections
- nitroblue tetrazolium test

Question 5

Nitroblue tetrazolium test

utility

Answer 5

turns blue if O2- is generated
turns blue (neg test) in MPO deficiency
no change (pos test) in NADPH oxidase deficiency

Question 6

Which organism is prominent in NADPH oxidase deficiency

Answer 6

Pseudomonas cepacia

anything oxidase positive really

Question 7

IL-10 function

Answer 7

anti-inflammatory

Question 8

TGF-β function

Answer 8

anti-inflammatory

Question 9

IL-8 function

Answer 9

neutrophil chemotaxis

Question 10

Are macrophages APCs?

Answer 10

yes