Neutrophil Arrival and Function

Question 1

What is in the Weibel-Palade bodies

Answer 1

W: vWF
P: P-selectin

Question 2

Types of selectins and source

Answer 2

P-selectin: endothelium

E-selectin

Question 3

P-selectin
source
induction via

Answer 3

from Weibel-Palade bodies

histamine-induced expression

Question 4

E-selectin

induction via

Answer 4

TNF and IL-1

Question 5

Selectins interact with what on neutrophils

Answer 5

integrins on neutrophils

Question 6

Leukocyte adhesion deficiency
defect
presentation

Answer 6

defect in CD18 subunit of integrins, neutrophils cannot migrate out of vasculature
presents as delayed separation of umbilical cord, because necrotic cells cannot be removed via neutrophils
also: recurrent infections w/o pus and elevated circulating neutrophil count (because do not migrate out)

Question 7

IL-8 function

Answer 7

neutrophil attraction

Question 8

Neutrophil attractants

Answer 8

IL-8
C5a
LTB4

Question 9

Functions of C3b and C5a

Answer 9

C3b: opsonin
C5a: neutrophil chemoattractant

Question 10

Chediak-Higashi syndrome
defect
presentation

Answer 10

microtubule defect --> impaired formation of phagolysosomes
presents as
-recurrent infections
-neutropenia (microtubules help mitosis)
-giant neutrophil granules
-defective primary hemostasis (abnormal granules)
-albinism (microtubules move melanin)
-peripheral neuropathy

Question 11

Function of phagolysosomes

Answer 11

produce free radicals to destroy phagocytosed material

ie bacteria, etc

Question 12

What regulates neutrophil rolling

Answer 12

selectins binding: P- and E-selectin binding sialyl Lewis X on leukocytes

Question 13

What do selectins bind (P- and E-selectin)

Answer 13

sialyl Lewis X on leukocytes

Question 14

What is sialyl Lewis X

Answer 14

on leukocytes
binds selectins (P- and E-selectin)