Neisseria and Moraxella catarrhalis Flashcards
Habitat of Neisseriaceae
Live on moist mucous membranes of man and other animals (oropharynx, genitourinary tract)
In ecological niches most do not produce disease
General growth requirements of the “pathogenic” species
Pathogenic species are fastidious
- Warmth (37C)
- Humidity
- CO2 (5-10%)
- Enriched media
General growth requirements of the
“non-pathogenic” species
- will grow on most primary media
- usually do not require CO2 or/or 37C
Purpose of a “Candle Jar”
It replaces a CO2 incubator
A capnophilic environment where CO2 is increased and O2 is decreased
(NOT ANAEROBIC)
Atmospheric conditions of a “Candle Jar”
3% carbon dioxide environment for culture plates
What are the 3 TYPES of culture media used to identify Neisseria and Moraxella?
- Non-enriched media
- Enriched media
- Selective, enriched media
What are the 3 selective, enriched media that contain antibiotics to inhibit normal flora in Neisseria and Moraxella?
- Modified Thayer Martin (MTM) - Chocolate
- Martin-Lewis (ML) - Chocolate
- New York City Medium (NYC) - horse blood
Group II Neisseria species
“commensals” - often grow on selective media
N. lactamica
M. catarrhalis (v)
N. cinerea (v)
Group III Neisseria species
"commensals" - No growth on selective N. mucosa N. sicca N. sublfava N. flavescens M. catarrhalis Others
Group I Neisseria species
“pathogenic” - growth on selective media
N. meningitidis
N. gonorrhoeae
______ and _______ require iron and compete with host by biding transferrin
Pathogenic Neisseria:
N. meningitidis and N. gonorrhoeae
Why can’t you get gonorrhoeae from the lab?
N. gonorrhoeae have pili in virulent strains.
Pili are lost upon subculture!
Two types of Oxidase Tests
Filterpaper method
Plate Method
Laboratory Identification Tests of Neisseria and Moraxella (10)
***impossible to memorize!!
- Probes
- Oxidase Test
- Catalase Test
- Superoxol Test (30% H2O2)
- Biochemicals
- MALDI-TOF
- FA, co-aggultination
- Nucleic Acid Amplification Tests (NAAT)
- Enzymatic procedures
- Growth requirements
ONPG tests detects what?
How long of a test?
Color for pos and neg?
- Detects late lactose fermentation
- (4 hour test)
- Pos Yellow, Neg Clear
N. gonorrhoeae
- disease caused?
agent of gonorrhea (eye infections, septicemia, pharyngitis, arthritis)
- anogenital v.s. non-genital infections
N gonorrhoeae
- How diagnosis is made
First day you get “suspicious or presumptive tests then you have to run confirmatory tests
First day “suspicious” or “presumptive” ways of identifying
N. gonorrhoeae (5)
- small, creamy, moist, translucent-brownish colonies
- Oxidase Positive
- Gram negative diplococci
- ONPG negative
- Superoxol (30% H2O2) - strongly positive
“Presumptive” positive plus confirmation of N. gonorrhoeae by one of the following…(5)
- MALDI-TOF
- Probe from colony
- Fluorescent antibody
- Rapid/CTA sugars (glucose only)
- Enzymatic or Co-aggultination
_______ confirming tests using different principles are required for isolates from patients less than 18 year old. Isolates must be ________.
Two and frozen
N gonorrhoeae
-Proper specimen collection
- dacron or rayon swabs are used (cotton or calcium alginate swabs inhibitory)
N gonorrhoeae
- Lab methods for identification
- GNID Slide Test performed only on male genital cultures
- GN diplococci must be see within cytoplasm of segs - Target Amplification Nucleic Acid Test
N. gonorrhoeae
- Proper transport
- Amies Transport medium
- Jembec Plate and Transgrow Bottles
May be normal respiratory flora (humans only)
Neisseria meningitidis
