Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Microbiology > Catalase Positive GPCs > Flashcards

Catalase Positive GPCs Flashcards

You may prefer our related Brainscape-certified flashcards:
Biology 101 flashcards
1
Q

Three genera of catalase positive GPCs

A
  1. Staphylococcus
  2. Micrococcus
  3. Rothia
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Purpose of CNA

A

for specimen with normal flora

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Purpose of Mannitol Salt Agar

A

For outbreaks

-Staphylococcus aureus is monnitol positive

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Two types of Catalase Test

A

Slide catalase and Plate catalase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

What happens during a Catalase Test?

A

H2O2 dissociates to H2 + O2

-positives will bubble

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Coagulase test purpose

A

Major test to separate Staphylococci into 2 categories

  • Coagulase positive Staph
  • Coagulase negative Staph (CoNS/CNS)
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

What is coagulase?

A

Enzyme that creates a thrombin like substance, clots human or rabbit PLASMA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Oxidation-Fermentation (OF) Test principle

A

One oiled tube and one not
Fermenter: yellow in oiled tube
Oxidizer: yellow in ONLY unoiled tuber
Fermenter: yellow in both tubes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Novobiocin/Furazolidone Susceptibility Test

A

Determine susceptibility/resistance to
antibiotic
-Zone of inhibition is measured after 18-24 hours

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Lysostaphin Test

A

Lysis Staphylococcus - tube will be clear as rather than turbine (no lysis)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Catalase Results for

  1. S. aureus
  2. S. epidermidis
  3. S. saprophyticus
  4. Micrococcus
A
  1. +
  2. +
  4. +
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Fermenter or Oxidizer?

  1. S. aureus
  2. S. epidermidis
  3. S. saprophyticus
  4. Micrococcus
A
  1. F
  2. F
  3. F
  4. O
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Susceptibility or Resistance to Furazolidone

  1. S. aureus
  2. S. epidermidis
  3. S. saprophyticus
  4. Micrococcus
A
  1. S
  2. S
  3. S
  4. R
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Susceptibility or Resistance to Novobiosin

  1. S. aureus
  2. S. epidermidis
  3. S. saprophyticus
  4. Micrococcus
A
  1. S
  2. S
  3. R
  4. Not Done
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Coagulase Test Result

  1. S. aureus
  2. S. epidermidis
  3. S. saprophyticus
  4. Mirococcus
A
  1. +
  2. -
  3. -
  4. -
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

SLIDE Coagulase

-Other names?

A

Clumping factor or bound coagulase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

TUBE Coagulase

-Other names?

A

Staphycoagulase or free coagulase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

Micrococcus

-common colonial characteristics

A

yellow/orange or “sticky”

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

Staphylococcus aureus

-common colonial characteristics

A

large, opaque, may be “golden”, usually Beta Hemolytic

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

Coagulase negative Staph

-common colonial characteristics

A

large, opaque, usually white, usually alpha or gamma hemolytic

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

Name the MAIN Staph. aureus infections

A 
Skin infection 
Wound infection 
Food Poising 
Scalded Skin Syndrome 
Toxic Shock Syndrome
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

Name the Coag neg Staph infections

A

Subacute Endocarditis

Catheter/Shunt Infections

23
Q

Name the S. saphrophyticus infection

A

Primarily Urinary Tract Infections

24
Q

Food poisoning

  • Infections and intoxications
    - timing of symptoms
    - stool sample needed?
A

Infections: 2 to 3 days for symptoms to show and yes stool sample needed

Intoxications: 2-8 hours for symptoms to show and no a stool would not be helpful

25
Q

List important patient symptoms/conditions that define Toxic Shock Syndrome

A

Fever
Rash
Desquamation
Shock (Hypotension)

26
Q

List S. aureus’ virulence factors

A
  • Protein A
  • Catalase
  • Coagulase
  • Hyaluronidase
  • DNase
  • Other: capsules, enterotoxins, exotoxins, beta-lactamases
27
Q

Protein A

A

1 cause of s. aureus virulence

Binds to Fc portion of IgG
Interferes with phagocytosis

28
Q

Staph. aureus food poisoning toxin is what type of toxin?

A

Enterotoxin which is a Exotoxin

29
Q

Staph. aureus’ scalded skin syndrome toxin is what type of toxin?

A

Exotoxin (exfoliatin)

30
Q

S. aureus Toxic Shock Syndrome is produced by what?

A

TSST-1

Toxic Shock Syndrome Toxin

31
Q

Define MSSA

A

Methicillin Susceptible Staph. aureus

32
Q

Define MRSA

A

Methicillin Resistant Staph. aureus

33
Q

Define VISA

A

Vancomycin Intermediate Staph. aureus

34
Q

Define VRSA

A

Vancomycin Resistant Staph. aureus

35
Q

Hospital acquired MRSA
v.s
Community acquired MRSA

A

HA-MRSA: highly resistant, requires isolation of patients

CA-MRSA: not as highly resistance, usually skin infections in otherwise healthy people

36
Q

Screening patients for MRSA

A
  • Oxacillin (or Vancomycin) screening plates

- PCR test for MecA gene (nasal swab)

37
Q

Screening colonies for MRSA

A
  • FDA approved automated methods (VITEK-II)

- Cefoxitin disk test - predicts Oxacillin resistance

38
Q

How do they detect staph carriers in hospital setting?

How do we protect from it?

A
  • Because it colonizes on hospital staff do nasal screens

- HANDWASHING

39
Q

Staphylococcus lugdunensis

  • How is it confused with s. aureus
  • How can they be differentiated
A
  • may have a positive slide coagulase test, may be beta hemolytic
  • it will be positive on the slide coagulase test so run the tube coagulase and it will be negative!
40
Q

Where is Staphylococcus saprophytic us most often found

A

Mostly unitary tract infections in sexually active, young females

41
Q

Rothia mucilaginosa

  • made of?
  • infection?
  • what does it look like?
A
  • Normal oral flora
  • Endocarditis and septicemia (esp. drug users and patients with malignancies
  • colonies often “sticky”
42
Q

Urinary Tract Infections

A

S. saprophyticus

43
Q

Dog Bites

A

S. intermedius

44
Q

Septicemia in drug users

A

Rothia mucilaginosa

45
Q

subacute endocarditis

A

Coagulase negative staphylococci CoNS

46
Q

acute endocarditis

A

S. aureus

47
Q

Toxic Shock

A

S. aureus

48
Q

scalded skin

A

S. aureus

49
Q

food poisoning

A

S. aureus

50
Q

Impetigo

A

S. aureus

51
Q

Celluitis

A

S. aureus

52
Q

Carbuncles/Furuncle

A

S. aureus

53
Q

Endocarditis

A

Rothia mucilaginosa

Microbiology (13 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions