Enterobacteriaceae PART2 Flashcards

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1
Q

Habitat and most common infection involving E. coli

A
  • Large bowel (#1)

- Urinary Tract Infection (#1 most common cause), gastroenteritis

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2
Q

Habitat and most common infection involving Shigella

A
  • NOT normal flora (#1) / always pathogenic

- penetrate epithelial cells of intestinal mucosa (blood, mucous, leukocytes)

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3
Q

Habitat and most common infection involving Salmonella

A
  • NOT normal flora (#2)

- Gastroenteritis (animals) and Septicemia

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4
Q

Habitat and most common infection involving Klebsiella pneumoniae

A
  • Bowel (#2)

- pneumonia (“current-jelly”) and nosocomial infections

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5
Q

Habitat and most common infection involving Citrobacter

A
  • GI?

- variety of infections, primarily urinary tract infections

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6
Q

Habitat and most common infection involving Serratia

A
  • GI, also respiratory flora

- Formerly strictly saprophytic now major nosocomial problem

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7
Q

Habitat and most common infection involving Enterobacter

A
  • GI?

- Variety of infections, esp. UTI

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8
Q

Habitat and most common infection involving Proteus and Morganella

A
  • GI?

- UTI…triple phosphate (struvite) crystals, renal stones

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9
Q

Habitat and most common infection involving Yersinia

A
  • Bubonic (lymph), pneumonic (lungs), septicemic (blood)

- Agent of the plague

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10
Q

Major source of infection for Enterohemorrhagic E. coli causing gastroenteritis

A

Hamburger, fruits, vegetables, water(pools), etc

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11
Q

Major source of infection for Salmonella causing gastroenteritis

A

Animals (chicken, turtles, iguanas ect), food, water

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12
Q

Major source of infection for causing gastroenteritis

A

?

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13
Q

Major source of infection for causing gastroenteritis

A

?

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14
Q

Major source of infection for Yersinia pestis

A
  • endemic in rodents and transmitted by insect vector, domestic cats to humans
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15
Q

Factors contributing to pathogenicity of EPEC and disease caused

A

Enteropathogenic E. coli

  • due to organism’s ability to adhere to cells in small intestine
  • Infant diarrhea
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16
Q

Factors contributing to pathogenicity of ETEC and disease caused

A

Enterotoxigenic E. coli

  • due to toxins (Heat labile toxin and Heat stable toxin)
  • Traveler’s Diarrhea (Montezuma’s Revenge)
17
Q

Factors contributing to pathogenicity of EIEC and disease caused

A

Enteroinvasive E. coli

  • due to ability to invade into cucosal cells of intestine not through to blood
  • Similar to Shigella (no specific disease caused)
18
Q

Factors contributing to pathogenicity of STEC, EHEC, VTEC and disease caused

A

Shiga Toxin, Enterohemorrhagic E. coli, Verotoxin

  • due to cytotoxins (verotoxins, and “shiga-like” toxins)
  • Leading cause of acute renal failure in children
19
Q

Formulate an approach for isolating/ identifying E. coli (O157:H7) in stool cultures

  • media?
  • biochemical recommendations?
  • typing recommendations?
A

(not sure of this answer but I think its important to know??)

  • use sorbitol MacConkey Agar (will be sorbitol neg)
  • run a MUG tes (will be MUG neg)
20
Q

Applications and advantages of shiga toxin testing on stool specimens

A
  • some sorbitol positive STEC strains identified (would be missed by culture)
  • assay will detect serotypes other than O157
21
Q

Compare and contrast the pathogenic mechanism involved in E. coli, Shigella, and Salmonellas gastroenteritis

A
22
Q

Typhoid Fever

  • recommended type of specimen
  • characteristics of the disease
  • specific characteristics for identification of pathogens
A
  • test positive in blood cultures
  • Man only host, invasive, high fever, carriers harbor in gallbladder
  • Serotyping required for identification: it is positive with Vi and group D antisera (may have to boil to get rxn with group D antisera)
23
Q

most common enteric agent of infection

- Urinary tract

A

E. coli

24
Q

most common enteric agent of infection

-abdominal wound

A

*

25
Q

most common enteric agent of infection

-newborn meningitis

A

*

26
Q

most common enteric agent of infection

- gastroenteritis

A

Salmonella

27
Q

most common enteric agent of infection

-HUS

A

Enterohemorrhagic E. Coli (STEC, EHEC, VTEC)

28
Q

most common enteric agent of infection

- pneumonia

A

Klebsiella pneumoniae

29
Q

most common enteric agent of infection

- typhoid fever

A

Salmonella Typhi

30
Q

most common enteric agent of infection

- plague

A

Yersinia pestis

31
Q

Name three Enterobacteriaceae that are ALWAYS considered pathogens, regardless of specimen site

A

Shigella, Salmonella