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Microbiology Module 2 '15-'16 > Mycobacteriology Practical > Flashcards

Mycobacteriology Practical Flashcards

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Biology 101 flashcards
1
Q

What are the characteristics of M. Haemophilium?

A

Requires Heme, optimal @30 degrees, isolated from immunocompromised

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2
Q

What nucleic acid probes are available?

A

MTB complex, M.avium-intracellulare complex, M. gordonae, and M kansasii

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3
Q

What are the characteristics of Nocardia sp.?

A

Appear powdery white due to aerial hyphae, Branching GPR, Partial acid fast

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4
Q

What is a mycobacterium that never produces pigment?

A

Nochromogen

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5
Q

What is mycobacterium that produces pigment after light exposure?

A

Photochromogen

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6
Q

What is a mycobacterium that produces pigment independently of light exposure?

A

Scotochromogen

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7
Q

What are nitrate test results for MTB and M bovis? What is added to a negative result?

A

MTB is pos (red)

Bovis is neg (no color change) Zinc dust is added to confirm test worked

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8
Q

What are niacin test results for MTB and M. Bovis?

A

MTB is pos (yellow), M. Bovis is neg (no color change)

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9
Q

What are characteristics of M. mucogenicum?

A

Rapid grower, wet colonies, Partially acid fast, nonchromogen

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10
Q

How is Kinyoun stain interpreted?

A

Red is pos, Blue is neg

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11
Q

What are the characteristics of Kinyoun stain?

A

Cold stain, Carbolfuchsin based, sold or liquid growth used

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12
Q

What mycobacterium demonstrates cording? What is it associated with?

A

MTB, virulence

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13
Q

What are the characteristics of M. marine?

A

Grows @28, skin infection with fish tanks or salt water, Photochromogen

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14
Q

What type of stain is Auramin-Rhodamine stain (AR said). What are its advantages and disadvantages?

A

Fluorochrome stain, performed directly on specimens
Ad=Most sensitive stain in AFB, Stains both viable and nonviable
Dis=Wont stain some rapid

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15
Q

What is the function of N-acetyl-L-cysteine?

A

mucolytic agent, frees organisms

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16
Q

What is the function of 2% NaOH?

A

Decontaminant to 5%

17
Q

What is the process of the N-acetyl-L-cysteine decontamination?

A

Sodium citrate binds to heavy metals, neutralizing buffer stops decontamination process, Phenol red indicates pH

18
Q

What are the incubation requirements for MAC w/o CV? How are results interpreted?

A

Ambient air, 28-30 for 7 days, used to ID rapid growers

Neg=no growth/scant growth POS is growth to end of line

19
Q

What does the NaCl tolerance test differentiate?

A

M. abscesses( >50 colonies= +) and M. Chelonae (

20
Q

What medium is used with the NaCl test and how is it incubated?

A

LJ with 5% NaCl, .1ml or light suspension @35-37 in CO2 for 4 weeks

21
Q

What are the incubation requirements for the tween-80 test? How are results interpreted?

A

35-37 ambient air for 10 days, Pink/red= nonpathogens=(+ result) No color change = pathogenic= (neg result)

22
Q

What does HPLC stand for? What is it used for?

A

High performance liquid chromatography, used to differentiate rapid growers based on mycelia acid contained in cell wall.

23
Q

What does a gram stain of mycobacteria look like?

A

Beaded or ghosted GPR, confused with strep

24
Q

What are the characteristics of Mycobacterium gordonae?

A

Commonly found in tap water, contaminate in lab, Scotochromogen

25
Q

What are the characteristics of MTB?

A

Rough and Buff, slow grower, nonchromogen, Prob available but only for complex ID with Niacin

26
Q

What are the characteristics of M. kansasii?

A

Probe, Photchromogen, candy cane striations

27
Q

What are the 6 media used in AFB lab? What do they ID?

A

MB bottle: Liquid for blood; BacT alert
MP Bottle: Liquid for all but blood; BacT alert
7H10 slant: Enriched Soild for all skin = incubate @30 too
Chocolate: for those that require heme @30
LJ: Biochemical testing
7H10 Plate: when isolation required

28
Q

What are the PPE in AFB lab?

A

Floves, impervious down, N95 or greater mask, foot covers

All checked at least once a year

29
Q

What are the characteristics of M. szulgai?

A

Scotochromogen @37, Photchromogen @25, nitrate positive, colonies either smooth or rough

Microbiology Module 2 '15-'16 (7 decks)

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