Anaerobes Intro Quiz Flashcards
What is an Obligate aerobe and examples?
Requires O2; Pseudomonas app. and Micrococcus spp.
What is a microaerophile and examples?
Reduced need for O2 and increased CO2 for growth, Campylobacter spp.
What is a facultative anaerobe and examples?
Most aerobes, under aerobic or anaerobic conditions, E. Coli and Staphylococcus aureus
What is an aerotolerant anaerobe and examples?
Best in anaerobic can tolerate O2 (tricky) Clostridium tertitum, C. cranes, C. histolyticum
What is a Moderate Obligate anaerobe and examples?
Most anaerobes, Tolerate moderate exposure to O2, Bacteroides Fragilis
What is a strict obligate anaerobe and examples?
Killed by O2, Clostridium novae and C. Haemolyticum
Identify sites of normal anaerobe flora.
Colon, Saliva, tooth scrapings, nasal washing, vaginal secretions, Skin, GI tract, Nose, Soil
What is an Endogenous infection?
Most common, comes from ones own flora
What is an Exogenous infection?
From soil or ingestion of contaminated food
What specimens are acceptable for anaerobe culture?
Needle aspirates at active site, Tissue, Blood, Deep respiratory, Stool only for C. Difficile, Normally sterile sites except Urine
What Specimens are UNacceptable for anaerobe culture?
Voided urine Upper respiratory tract, Upper respiratory (sputum included), Stool except for C. Difficile, Vaginal.Cervical, Surface wounds or outside ulcers
Why are aspirated samples preferred over swabs?
Go directly into an acerbic environment
What are the 3 requirements for anaerobe media?
Vitamin K, Hemin and Yeast Extract
What are the conditions necessary for anaerobic media?
Stored in a holding chamber for one hour or placed in the anaerobic chamber immediately.
Why is ABAP used?
Grows almost everything (non-selective)
What is PEA used?
Supports both GP and GN anaerobes but inhibits GNR facultative anaerobes. INHIBITS swarming of Proteus
What is PV/KV/LKV used?
Selective for GN
Why is LKV used?
Selective for GN contains Laked Blood
Why is BBE used?
Supports Bacteroides due to high bile, turns black with esculin hydrolysis
Grows in aerobic ambient air and CO2 but NOT Anaerobically
Obligate aerobe
Grows in aerobically CO2 but NOT ambient or Anaerobically
Capnophilic Aerobe
Grows in ambient, aerobic CO2 and anaerobically
Facultative Anaerobe
Grows anaerobically but NOT ambient or aerobic CO2
Obligate anaerobe
