Antimicrobials lab

Question 1

If there is no growth in all wells, what do you report?

Answer 1

greater than, resistant

Question 2

If there IS growth in all the wells, what do you report?

Answer 2

Less than OR equal to smallest value tested, susceptible

Question 3

What is the purpose of the ESBL test?

Answer 3

Detect presence of extended spectrum beta lactamase enzymes

Question 4

What are the 4 disks used in the ESBL test?

Answer 4

Cefotaxime, cefotazime + clavelanic acid, Ceftazidime, Ceftazidime +clavelanic acid

Question 5

What zone size indicates a positive result in ESBL?

Answer 5

greater than or EQUAL to 5mm

Question 6

What is the purpose of the D-test?

Answer 6

To detect inducible resistance to clindamycin

Question 7

What are the two disks used in the D-test?

Answer 7

Clindamycin and Erythromycin

Question 8

What is a positive D-test?

Answer 8

“d” around the Clindamycin (CC) disk

Question 9

What is the purpose of the cefoxitin screen?

Answer 9

See if isolate produces the meCA gene (MRSA or MSSA)

Question 10

What is a positive cefoxitin?

Answer 10

Less than OR equal to 21mm

Question 11

What is a negative cefoxitin?

Answer 11

Greater than or equal to 22mm

Question 12

What is the purpose of the Hodge test?

Answer 12

To detect presence of carbapenmases

Question 13

What does CRE stand for?

Answer 13

Carbapenem Resistance

Question 14

What organism is streaked all over Hodge plate?

Answer 14

e. coli

Question 15

What disk is placed in the middle of the Hodge test?

Answer 15

meropenem disk

Question 16

What is a positive and negative beta-lactamase disk test look like?

Answer 16

Red (S. aurous) is positive and yellow (H. influenza) is negative