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Microbiology Module 2 '15-'16 > CSF Cultures > Flashcards

CSF Cultures Flashcards

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1
Q

Define subdural taps

A

Between dura matter and arachnid membrane

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2
Q

Define Ventricular aspiration

A

Needle goes into one of the ventricles of the brain

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3
Q

Define lumbar puncture

A

Subarachnoid space in lower back

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4
Q

Define acute meningitis

A

Fever, stiff neck headache, change in mental status, logic, Increase in segs

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5
Q

Define Chronic Meningitis

A

Immunocompromised, may or amy not have symptoms of above, vomiting nausea longer duration and usually lymphs

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6
Q

Define aseptic meningitis

A

usually viral meningitis, not truly aseptic because of virus cause

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7
Q

Define encephalitis

A

Inflammation of the brain

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8
Q

Define meningoencephalitis

A

Meningitis and encephalitis

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9
Q

What are the symptoms of meningitis?

A

Photophobia, 3-7 days after exposure, reduced consciousness, Brudzinski and Kerning signs

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10
Q

What bacteria is most common in neonates?

A

Beta Streptococcus, S. agalactiae

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11
Q

What bacteria is most common with Children?

A

H. influenzae (Type B) (S. pneumoniae, and N. meningitis)

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12
Q

What bacteria is most common in Young adults?

A

N. Meningitis

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13
Q

What bacteria is most common in older adults?

A

S. Pneumoniae (listeria)

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14
Q

What bacteria is most common in immunocompromised?

A

Fungal

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15
Q

How are CSF cultures stored?

A

Room temp, never fridge

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16
Q

What if you have more than 1 ml?

A

Centrifuge, our off and resuspend

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17
Q

What do you plate and do with a CSF culture?

A

2 Slides must correlate, CHOC, BLD, and THIO

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18
Q

What additional plates do you set up for brain accesses?

A

ANA BLD

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19
Q

additional for clustering GPC

A

Coagulase tube

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20
Q

Additional for lancet GPC

A

optochin

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21
Q

Additional for GNR

A

MAC

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22
Q

Additional for GNC

A

Martin Lewis

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23
Q

What additional testing is needed if you can not find bacteria

A

Acridine orange, glucose and protein

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24
Q

What are the normal Glucose and Protein for adults?

A

15-45mg/dl and 60-70%

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25
Q

What is the normal protein for premature?

A
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26
Q

What is the normal protein for infants?

A
27
Q

What are the glucose and protein levels for bacterial meningitis? What cells are present?

A

> 45 and

28
Q

What are the glucose and protein levels for viral meningitis? What cells are present?

A

between 45 and 100m about 50, lymphs

29
Q

What are the glucose and protein levels for fungal meningitis? What cells are present?

A

45mg, Lymphs and monos

30
Q

What are the glucose and protein levels for tubercular meningitis? What cells are present?

A

45mg, Early neutrophils and late lymphs

31
Q

What is the most common bacteria for brain accesses?

A

Normal mouth flora (anaerobe) usually mixed culture

32
Q

What are the most common bacteria for shunt infections?

A

Coat neg staph, Skin flora

33
Q

Define Ventricular aspiration

A

Needle goes into one of the ventricles of the brain

34
Q

Define lumbar puncture

A

Subarachnoid space in lower back

35
Q

Define acute meningitis

A

Fever, stiff neck headache, change in mental status, logic, Increase in segs

36
Q

Define Chronic Meningitis

A

Immunocompromised, may or amy not have symptoms of above, vomiting nausea longer duration and usually lymphs

37
Q

Define aseptic meningitis

A

usually viral meningitis, not truly aseptic because of virus cause

38
Q

Define encephalitis

A

Inflammation of the brain

39
Q

Define meningoencephalitis

A

Meningitis and encephalitis

40
Q

What are the symptoms of meningitis?

A

Photophobia, 3-7 days after exposure, reduced consciousness, Brudzinski and Kerning signs

41
Q

What bacteria is most common in neonates?

A

Beta Streptococcus, S. agalactiae

42
Q

What bacteria is most common with Children?

A

H. influenzae (Type B) (S. pneumoniae, and N. meningitis)

43
Q

What bacteria is most common in Young adults?

A

N. Meningitis

44
Q

What bacteria is most common in older adults?

A

S. Pneumoniae (listeria)

45
Q

What bacteria is most common in immunocompromised?

A

Fungal

46
Q

How are CSF cultures stored?

A

Room temp, never fridge

47
Q

What if you have more than 1 ml?

A

Centrifuge, our off and resuspend

48
Q

What do you plate and do with a CSF culture?

A

2 Slides must correlate, CHOC, BLD, and THIO

49
Q

What additional plates do you set up for brain accesses?

A

ANA BLD

50
Q

additional for clustering GPC

A

Coagulase tube

51
Q

Additional for lancet GPC

A

optochin

52
Q

Additional for GNR

A

MAC

53
Q

Additional for GNC

A

Martin Lewis

54
Q

What additional testing is needed if you can not find bacteria

A

Acridine orange, glucose and protein

55
Q

What are the normal Glucose and Protein for adults?

A

15-45mg/dl and 60-70%

56
Q

Define subdural taps

A

Between dura matter and arachnid membrane

57
Q

What is the normal protein for premature?

A
58
Q

What is the normal protein for infants?

A
59
Q

What are the glucose and protein levels for bacterial meningitis? What cells are present?

A

> 45 and

60
Q

What are the glucose and protein levels for viral meningitis? What cells are present?

A

between 45 and 100m about 50, lymphs

61
Q

What are the glucose and protein levels for fungal meningitis? What cells are present?

A

45mg, Lymphs and monos

62
Q

What are the glucose and protein levels for tubercular meningitis? What cells are present?

A

45mg, Early neutrophils and late lymphs

63
Q

What is the most common bacteria for brain accesses?

A

Normal mouth flora (anaerobe) usually mixed culture

64
Q

What are the most common bacteria for shunt infections?

A

Coat neg staph, Skin flora

Microbiology Module 2 '15-'16 (7 decks)

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