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Microbiology Module 2 '15-'16 > CSF Cultures > Flashcards

CSF Cultures Flashcards

1
Q

Define subdural taps

A

Between dura matter and arachnid membrane

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2
Q

Define Ventricular aspiration

A

Needle goes into one of the ventricles of the brain

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3
Q

Define lumbar puncture

A

Subarachnoid space in lower back

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4
Q

Define acute meningitis

A

Fever, stiff neck headache, change in mental status, logic, Increase in segs

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5
Q

Define Chronic Meningitis

A

Immunocompromised, may or amy not have symptoms of above, vomiting nausea longer duration and usually lymphs

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6
Q

Define aseptic meningitis

A

usually viral meningitis, not truly aseptic because of virus cause

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7
Q

Define encephalitis

A

Inflammation of the brain

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8
Q

Define meningoencephalitis

A

Meningitis and encephalitis

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9
Q

What are the symptoms of meningitis?

A

Photophobia, 3-7 days after exposure, reduced consciousness, Brudzinski and Kerning signs

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10
Q

What bacteria is most common in neonates?

A

Beta Streptococcus, S. agalactiae

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11
Q

What bacteria is most common with Children?

A

H. influenzae (Type B) (S. pneumoniae, and N. meningitis)

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12
Q

What bacteria is most common in Young adults?

A

N. Meningitis

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13
Q

What bacteria is most common in older adults?

A

S. Pneumoniae (listeria)

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14
Q

What bacteria is most common in immunocompromised?

A

Fungal

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15
Q

How are CSF cultures stored?

A

Room temp, never fridge

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16
Q

What if you have more than 1 ml?

A

Centrifuge, our off and resuspend

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17
Q

What do you plate and do with a CSF culture?

A

2 Slides must correlate, CHOC, BLD, and THIO

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18
Q

What additional plates do you set up for brain accesses?

A

ANA BLD

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19
Q

additional for clustering GPC

A

Coagulase tube

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20
Q

Additional for lancet GPC

A

optochin

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21
Q

Additional for GNR

A

MAC

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22
Q

Additional for GNC

A

Martin Lewis

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23
Q

What additional testing is needed if you can not find bacteria

A

Acridine orange, glucose and protein

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24
Q

What are the normal Glucose and Protein for adults?

A

15-45mg/dl and 60-70%

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25
What is the normal protein for premature?
26
What is the normal protein for infants?
27
What are the glucose and protein levels for bacterial meningitis? What cells are present?
>45 and
28
What are the glucose and protein levels for viral meningitis? What cells are present?
between 45 and 100m about 50, lymphs
29
What are the glucose and protein levels for fungal meningitis? What cells are present?
45mg, Lymphs and monos
30
What are the glucose and protein levels for tubercular meningitis? What cells are present?
45mg, Early neutrophils and late lymphs
31
What is the most common bacteria for brain accesses?
Normal mouth flora (anaerobe) usually mixed culture
32
What are the most common bacteria for shunt infections?
Coat neg staph, Skin flora
33
Define Ventricular aspiration
Needle goes into one of the ventricles of the brain
34
Define lumbar puncture
Subarachnoid space in lower back
35
Define acute meningitis
Fever, stiff neck headache, change in mental status, logic, Increase in segs
36
Define Chronic Meningitis
Immunocompromised, may or amy not have symptoms of above, vomiting nausea longer duration and usually lymphs
37
Define aseptic meningitis
usually viral meningitis, not truly aseptic because of virus cause
38
Define encephalitis
Inflammation of the brain
39
Define meningoencephalitis
Meningitis and encephalitis
40
What are the symptoms of meningitis?
Photophobia, 3-7 days after exposure, reduced consciousness, Brudzinski and Kerning signs
41
What bacteria is most common in neonates?
Beta Streptococcus, S. agalactiae
42
What bacteria is most common with Children?
H. influenzae (Type B) (S. pneumoniae, and N. meningitis)
43
What bacteria is most common in Young adults?
N. Meningitis
44
What bacteria is most common in older adults?
S. Pneumoniae (listeria)
45
What bacteria is most common in immunocompromised?
Fungal
46
How are CSF cultures stored?
Room temp, never fridge
47
What if you have more than 1 ml?
Centrifuge, our off and resuspend
48
What do you plate and do with a CSF culture?
2 Slides must correlate, CHOC, BLD, and THIO
49
What additional plates do you set up for brain accesses?
ANA BLD
50
additional for clustering GPC
Coagulase tube
51
Additional for lancet GPC
optochin
52
Additional for GNR
MAC
53
Additional for GNC
Martin Lewis
54
What additional testing is needed if you can not find bacteria
Acridine orange, glucose and protein
55
What are the normal Glucose and Protein for adults?
15-45mg/dl and 60-70%
56
Define subdural taps
Between dura matter and arachnid membrane
57
What is the normal protein for premature?
58
What is the normal protein for infants?
59
What are the glucose and protein levels for bacterial meningitis? What cells are present?
>45 and
60
What are the glucose and protein levels for viral meningitis? What cells are present?
between 45 and 100m about 50, lymphs
61
What are the glucose and protein levels for fungal meningitis? What cells are present?
45mg, Lymphs and monos
62
What are the glucose and protein levels for tubercular meningitis? What cells are present?
45mg, Early neutrophils and late lymphs
63
What is the most common bacteria for brain accesses?
Normal mouth flora (anaerobe) usually mixed culture
64
What are the most common bacteria for shunt infections?
Coat neg staph, Skin flora

Microbiology Module 2 '15-'16 (7 decks)

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