Muscle and Contractile proteins (BAKER) Flashcards
There are many myosin types with similiar (blank) but with differences in (blank) which correspond to differences in cargo and regulation.
motor domains
tails
Why type of myosin is associated with muscle myopathies?
type II
What type of myosin is associated with griscelli syndrome?
type V
What type of myosin is associated with hearing loss?
Type VI and VII
What is the function of myosin IIa?
involved in cell division
What is the function of myosin V?
involved in melanosome transport and certain neurological function
What is Griscellis syndrome and what causes it?
myosin Va point mtation leads to hypopigmentatiosn and neurological defects
What is the function of myosin VI and myosin VII and what is the disease associated with this?
maintains organization of actin-filled stereocilia
Mutations to myosin VI and VII associated with hearing loss. and usher syndrome (VII)
What is the structure of myosin II?
it is a dimer with motor domains and tails associated with heavy chains and 2 light chains (essential and regulatory)
with an active site and actin binding site
What is essential for myosin function?
coordination b/w actin binding site and active site
To generate ATP you need (blank) site.
actin binding site
all myosins are (blank) motors
actin-based motors
all but (blank) are plus-end directed myosin.
myosin VI
Actin is a long polymer structure, the polymer is (blank) allowing for directional muscle contraction.
polar
What are the sources of ATP in muscle and tell me the order that they are utilized.
ATP> creatinine>glycogen> lipolysis
with anaerobic respiration what do you get?
lactic acid and 2 ATPs
With aerobic respiration what do you get?
36 ATPs, CO2 and water and has myoglobin stores for oxygen
Why are aerobic cells red and what surrounds them?
aerobic cuz of myoglobin
vascularization surrounds it
Explain how muscle contraction works with actin and myosin
myosin is locked on actin in rigor configuration-> ATP binds and causes conformation on actin binding site->head displaces-> hydrolysis of ATP-> myosin binds to new site on actin filament-> release of phosphate-> power stroke and loss of ADP-> back to rigor state
Which has a bigger lever arm, myosin II or myosin V?
myosin V?
What is the mechanism of movement (working step) of actin and myosin?
lever arm otation
(blank) is a high (greater than 50%) duty ratio “processive” motor. i.e one head (motor) must be bound for 50% of its ATPase cycle
myosin V
(blank) transports vesicles in cells
myosin V
(blank) is a low duty ratio (less than 10%) motor
myosin II
What is this:
long neck (6IQ)
organelle motor
functional unit: two heads
myosin V
What is this:
shot neck (2IQ)
drives muscle contraction
functional unit: ~20 heads
myosin II
(blank) are far from processive
muscle myosin
How come some myosins can be processive and others cant/
the duty ratio tells us how long a myosin can hang onto an actin and if you have a very short duty ratio, then the myosin will just fall off and you cant processively (move like monkey bars).
How does muscle myosin (myosin II) contract?
by forming a thick filament with about 20 muscle heads and creates a successive contraction on actin
Many (blank) molecules are required to propel actin filament
myosin II molecules
In muscle, myosin II molecules are assembled into a (blank)
thick filament
(blank) form by association of hydrophobic regions in the tail.
filaments
(blank) is the fundamental contractile unit in muscle.
muscle sarcomere
The (blank) contracts when myosin thick filaments and actin thin filaments slide past each other.
sarcomere
(blank) and (blank)cap ends o actin to keep filament length constant.
CapZ and tropomodulin
(blank) contains alpha-actinin an other proteins that stably join sarcomeres.
Z disc
(blank) maintains thick filament position in the sarcomere.
Titin
(blank) sets the length of the thin filament
nebulin
What are the two types of muscle regulation?
thin filament (skeletal and cardiac) thick filament (smooth muscle)
Explain smooth muscle regulation?
phosphorylation= contraction
What activates myosin light chain kinase?
calcium-calmodulin
(blank) wraps around actin filaments blocking myosin binding sites on actin.
tropomyosin
(blank) binding to troponin C results in tropomyosin movement away from myosin binding sites.
calcium